Flow-cytometry-based protocols for human blood/marrow immunophenotyping with minimal sample perturbation

Laura G Rico; Roser Salvia; Michael D Ward; Jolene A Bradford; Jordi Petriz

doi:10.1016/j.xpro.2021.100883

Flow-cytometry-based protocols for human blood/marrow immunophenotyping with minimal sample perturbation

STAR Protoc. 2021 Oct 11;2(4):100883. doi: 10.1016/j.xpro.2021.100883. eCollection 2021 Dec 17.

Authors

Laura G Rico¹, Roser Salvia¹, Michael D Ward², Jolene A Bradford², Jordi Petriz¹

Affiliations

¹ Functional Cytomics Lab, Josep Carreras Leukaemia Research Institute, ICO-Hospital Germans Trias i Pujol, Universitat Autònoma de Barcelona, Badalona, Barcelona, Spain.
² Thermo Fisher Scientific, Eugene, OR, USA.

Abstract

This protocol provides instructions to improve flow cytometry analysis of marrow/peripheral blood cells by avoiding erythrolytic solutions, density gradients, and washing steps. We describe two basic approaches for identifying cell surface antigens with minimal sample perturbation, which have been successfully used to identify healthy and pathologically rare cells. The greatest advantage of these approaches is that they minimize the unwanted effect caused by sample preparation, allowing for improved study of live cells at the point of analysis. For complete details on the use and execution of this protocol, please refer to Petriz et al. (2018).

Keywords: Cancer; Clinical Protocol; Flow Cytometry/Mass Cytometry; Health Sciences; Immunology; Stem Cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Blood Cells / cytology*
Bone Marrow Cells / cytology*
Flow Cytometry / methods*
Humans
Immunophenotyping / methods*