Regulation of Cardiac-Specific Proteins Expression by Moderate-Intensity Aerobic Exercise Training in Mice With Myocardial Infarction Induced Heart Failure Using MS-Based Proteomics

Shouling Mi; Hao Jiang; Lei Zhang; Zhonglei Xie; Jingmin Zhou; Aijun Sun; Hong Jin; Junbo Ge

doi:10.3389/fcvm.2021.732076

Regulation of Cardiac-Specific Proteins Expression by Moderate-Intensity Aerobic Exercise Training in Mice With Myocardial Infarction Induced Heart Failure Using MS-Based Proteomics

Front Cardiovasc Med. 2021 Oct 8:8:732076. doi: 10.3389/fcvm.2021.732076. eCollection 2021.

Authors

Shouling Mi^{1

2}, Hao Jiang^{1

2}, Lei Zhang³, Zhonglei Xie^{1

2}, Jingmin Zhou^{1

2}, Aijun Sun^{1

2

3}, Hong Jin^{3

4}, Junbo Ge^{1

2

3}

Affiliations

¹ Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai, China.
² Shanghai Institute of Cardiovascular Diseases, Shanghai, China.
³ Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
⁴ Stomatological Hospital, Fudan University, Shanghai, China.

Abstract

This study aims to systematically reveal the changes in protein levels induced by regular exercise in mice with ischemic-induced heart failure (HF). Aerobic exercise training for the ischemic-induced HF mice lasted for 4 weeks and then we used the liquid chromatography-mass spectrometry method to identify and quantify the protein profile in the myocardium of mice. As a whole, 1,304 proteins (597 proteins up-regulated; 707 proteins down-regulated) were differentially expressed between the exercise group and the sedentary group, including numerous proteins related to energy metabolism. The significant alteration of the component (E1 component subunit alpha and subunit beta) and the activity-regulating enzyme (pyruvate dehydrogenase kinase 2 and pyruvate dehydrogenase kinase 4) of pyruvate dehydrogenase complex and poly [ADP-ribose] polymerase 3, a nicotinamide adenine dinucleotide(+)-consuming enzymes, was further verified in targeted analysis. Generally, this proteomics profiling furnishes a systematic insight of the influence of aerobic exercise on HF.

Keywords: aerobic exercise; heart failure; mice; myocardial infarction; proteomics.