Three-liquid-phase salting-out extraction of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)-rich oils from Euphausia superba

Liaqat Zeb; Xin-Nan Teng; Muhammad Shafiq; Shu-Chang Wang; Zhi-Long Xiu; Zhi-Guo Su

doi:10.1002/elsc.202000098

Three-liquid-phase salting-out extraction of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)-rich oils from Euphausia superba

Eng Life Sci. 2021 Jul 26;21(10):666-682. doi: 10.1002/elsc.202000098. eCollection 2021 Oct.

Authors

Liaqat Zeb¹, Xin-Nan Teng¹, Muhammad Shafiq¹, Shu-Chang Wang¹, Zhi-Long Xiu¹, Zhi-Guo Su²

Affiliations

¹ School of Bioengineering Dalian University of Technology Dalian P. R. China.
² State Key Laboratory of Biochemical Engineering Institute of Process Engineering Chinese Academy of Sciences Beijing P. R. China.

Abstract

The TLPSOES parameters were optimized by response surface methodology using Box-Behnken design, which were 16.5% w/w of ammonium citrate, 17.5% w/w of ethanol, and 46% w/w of n-hexane at 70 min of stirring time. Under optimized conditions the extraction efficiency attained was 90.91 ± 0.97% of EPA, 90.02 ± 1.04% of DHA, and 91.85 ± 1.11% of KO in the top n-hexane phase. The highest extraction efficiency of proteins and flavonoids, i.e. 88.34 ± 1.35% and 79.67 ± 1.13%, was recorded in the solid interface and ethanol phase, respectively. The KO extracted by TLPSOES system consisted of lowest fluoride level compared to the conventional method and whole wet krill biomass. The TLPSOES is a potential candidate for nutraceutical industry of KO extraction from wet krill biomass.

Keywords: Antarctic krill; docosahexaenoic acid; eicosapentaenoic acid; extraction efficiency; fluoride; three‐liquid‐phase salting‐out extraction system.