Pseudevernia furfuracea inhibits migration and invasion of colorectal carcinoma cell lines

Dragana S Šeklić; Milena M Jovanović; Katarina D Virijević; Jelena N Grujić; Marko N Živanović; Snežana D Marković

doi:10.1016/j.jep.2021.114758

Pseudevernia furfuracea inhibits migration and invasion of colorectal carcinoma cell lines

J Ethnopharmacol. 2022 Feb 10:284:114758. doi: 10.1016/j.jep.2021.114758. Epub 2021 Oct 21.

Authors

Dragana S Šeklić¹, Milena M Jovanović², Katarina D Virijević³, Jelena N Grujić⁴, Marko N Živanović⁵, Snežana D Marković⁶

Affiliations

¹ Department of Natural Sciences, Institute for Information Technologies Kragujevac, University of Kragujevac, Serbia. Electronic address: ddjacic@yahoo.com.
² Department of Biology and Ecology, Faculty of Science, University of Kragujevac, Kragujevac, Serbia. Electronic address: milena.jovanovic@pmf.kg.ac.rs.
³ Department of Natural Sciences, Institute for Information Technologies Kragujevac, University of Kragujevac, Serbia. Electronic address: msc.katarina.virijevic@gmail.com.
⁴ Department of Natural Sciences, Institute for Information Technologies Kragujevac, University of Kragujevac, Serbia. Electronic address: jelena.grujic2@aiesec.net.
⁵ Department of Natural Sciences, Institute for Information Technologies Kragujevac, University of Kragujevac, Serbia. Electronic address: zivanovicmkg@gmail.com.
⁶ Department of Biology and Ecology, Faculty of Science, University of Kragujevac, Kragujevac, Serbia. Electronic address: snezana.markovic@pmf.kg.ac.rs.

PMID: 34688797
DOI: 10.1016/j.jep.2021.114758

Abstract

Ethnopharmacological relevance: Pseudevernia furfuracea (L.) Zopf is common lichen species, traditionally used worldwide in treating various medical conditions, among which are intestinal issues and cancer. Most studies are focused mainly on cytotoxic potential of lichens, whilst their antimigratory and antiinvasive properties are often disregarded. Migration and invasion of cancer cells are pivotal processes in cancer metastasis, wherein cancer cells are able to migrate individually or in form of a coherent mass. One of successful strategies in anticancer treatments is targeting Wnt/β-catenin signal pathway, that is aberrantly activated in colorectal carcinoma, as well as lowering level of migratory/invasive markers.

Aim of the study: Present study aimed to show antimigratory/invasive potential of Pseudevernia furfuracea methanol extract on HCT-116 and SW-480 colorectal carcinoma cell lines and to elucidate possible mechanism of its action.

Materials and methods: Collective cell migration was assessed by Wound healing assay and single cell migration in real time by RTCA method. Analysis of anti- and promigratory protein expression was performed using immunofluorescent staining. Additionally, gene expression of antimigratory/promigratory and invasive (E-cadherin, β-catenin, N-cadherin, Vimentin, Snail and MMP-9) markers were investigated by qRT-PCR method. Concentration of MMP-9 was determined colorimetrically by ELISA test.

Results: P. furfuracea extract was able to suppress both collective and single cancer cell migration, by inhibiting expression of promigratory/invasive markers and possibly re-establishing cell-cell adhesions. The present study indicates at P. furfuracea as effective antimigratory treatment, and HCT-116 cells were proved to be a more sensitive cell line to applied treatment.

Conclusions: This lichen species is a promising candidate for application in treatment of cancer in order to prevent metastasis.

Keywords: Collective cell migration; Immunofluorescence; Invasion; Natural products; Pseudevernia furfuracea.

MeSH terms

Antineoplastic Agents / chemistry*
Antineoplastic Agents / pharmacology*
Cell Line, Tumor
Cell Movement
Chemical Fractionation
Colorectal Neoplasms / genetics
Colorectal Neoplasms / metabolism*
Humans
Lichens*
Medicine, Traditional
Wound Healing

Substances

Antineoplastic Agents