A forebrain neural substrate for behavioral thermoregulation

Abstract

Thermoregulatory behavior is a basic motivated behavior for body temperature homeostasis. Despite its fundamental importance, a forebrain region or defined neural population required for this process has yet to be established. Here, we show that Vgat-expressing neurons in the lateral hypothalamus (LH^Vgat neurons) are required for diverse thermoregulatory behaviors. The population activity of LH^Vgat neurons is increased during thermoregulatory behavior and bidirectionally encodes thermal punishment and reward (P&R). Although this population also regulates feeding and caloric reward, inhibition of parabrachial inputs selectively impaired thermoregulatory behaviors and encoding of thermal stimulus by LH^Vgat neurons. Furthermore, two-photon calcium imaging revealed a subpopulation of LH^Vgat neurons bidirectionally encoding thermal P&R, which is engaged during thermoregulatory behavior, but is largely distinct from caloric reward-encoding LH^Vgat neurons. Our data establish LH^Vgat neurons as a required neural substrate for behavioral thermoregulation and point to the key role of the thermal P&R-encoding LH^Vgat subpopulation in thermoregulatory behavior.

Keywords: behavioral thermoregulation; lateral hypothalamus; motivated behavior; parabrachial nucleus; reward and punishment; thermal homeostasis; thermal reward; thermoregulatory behavior; two-photon calcium imaging; vesicular GABA transporter.