Bioinformatics in Immunoglobulin Glycosylation Analysis

Frédérique Lisacek; Kathirvel Alagesan; Catherine Hayes; Steffen Lippold; Noortje de Haan

doi:10.1007/978-3-030-76912-3_6

Bioinformatics in Immunoglobulin Glycosylation Analysis

Exp Suppl. 2021:112:205-233. doi: 10.1007/978-3-030-76912-3_6.

Authors

Frédérique Lisacek^{1

2

3}, Kathirvel Alagesan⁴, Catherine Hayes^{5

6}, Steffen Lippold⁷, Noortje de Haan⁸

Affiliations

¹ Proteome Informatics Group, SIB Swiss Institute of Bioinformatics, Geneva, Switzerland. frederique.lisacek@sib.swiss.
² Computer Science Department, University of Geneva, Geneva, Switzerland. frederique.lisacek@sib.swiss.
³ Section of Biology, University of Geneva, Geneva, Switzerland. frederique.lisacek@sib.swiss.
⁴ Max Planck Unit for the Science of Pathogens, Berlin, Germany.
⁵ Proteome Informatics Group, SIB Swiss Institute of Bioinformatics, Geneva, Switzerland.
⁶ Computer Science Department, University of Geneva, Geneva, Switzerland.
⁷ Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands.
⁸ Copenhagen Center for Glycomics, University of Copenhagen, Copenhagen, Denmark.

PMID: 34687011
DOI: 10.1007/978-3-030-76912-3_6

Abstract

Analytical methods developed for studying immunoglobulin glycosylation rely heavily on software tailored for this purpose. Many of these tools are now used in high-throughput settings, especially for the glycomic characterization of IgG. A collection of these tools, and the databases they rely on, are presented in this chapter. Specific applications are detailed in examples of immunoglobulin glycomics and glycoproteomics data processing workflows. The results obtained in the glycoproteomics workflow are emphasized with the use of dedicated visualizing tools. These tools enable the user to highlight glycan properties and their differential expression.

Keywords: Database; Glycoinformatics; Glycomics; Glycoproteomics; Software; Workflow.

MeSH terms

Computational Biology*
Glycomics
Glycoproteins* / genetics
Glycoproteins* / metabolism
Glycosylation
Immunoglobulins

Substances

Glycoproteins
Immunoglobulins