Insulin stimulates β-alanine uptake in skeletal muscle cells in vitro

Lívia Santos; L S Gonçalves; Shirin Bagheri-Hanei; Gabriella Berwig Möller; Craig Sale; Ruth M James; Guilherme Giannini Artioli

doi:10.1007/s00726-021-03090-9

Insulin stimulates β-alanine uptake in skeletal muscle cells in vitro

Amino Acids. 2021 Nov;53(11):1763-1766. doi: 10.1007/s00726-021-03090-9. Epub 2021 Oct 21.

Authors

Lívia Santos¹, L S Gonçalves², Shirin Bagheri-Hanei³, Gabriella Berwig Möller², Craig Sale¹, Ruth M James¹, Guilherme Giannini Artioli⁴

Affiliations

¹ Musculoskeletal Physiology Research Group, Sport, Health and Performance Enhancement Research Centre, Nottingham Trent University, Nottingham, UK.
² Applied Physiology and Nutrition Research GroupRheumatology DivisionFaculdade de Medicina FMUSPEscola de Educação Física E Esporte, Universidade de São Paulo, São Paulo, SP, Brazil.
³ College of Engineering and Physical Science, Aston University, Birmingham, B4 7ET, UK.
⁴ Department of Life Sciences, Manchester Metropolitan University, John Dalton Building, Manchester, M1 5GD, UK. G.Giannini.Artioli@mmu.ac.uk.

Abstract

We evaluated whether insulin could stimulate β-alanine uptake by skeletal muscle cells in vitro. Mouse myoblasts (C2C12) (n = 3 wells per condition) were cultured with β-alanine (350 or 700 µmol·L^-1), with insulin (100 µU·mL^-1) either added to the media or not. Insulin stimulated the β-alanine uptake at the lower (350 µmol·L^-1) but not higher (700 µmol·L^-1) β-alanine concentration in culture medium, indicating that transporter saturation might blunt the stimulatory effects of insulin.

Keywords: Carnosine; Insulin; Taurine transporter; β-alanine.

MeSH terms

Animals
Biological Transport
Cell Line
Insulin / analysis
Insulin / metabolism*
Mice
Muscle Fibers, Skeletal / cytology
Muscle Fibers, Skeletal / metabolism*
Myoblasts / metabolism*
beta-Alanine / metabolism*

Substances

Insulin
beta-Alanine

Abstract

MeSH terms

Substances

Grants and funding