Chromatin Immunoprecipitation Protocol for Circadian Clock Proteins

Methods Mol Biol. 2022:2398:135-150. doi: 10.1007/978-1-0716-1912-4_12.

Abstract

Chromatin immunoprecipitation, or ChIP, is a powerful experimental technique for probing protein-DNA interactions in vivo. This assay can be used to investigate the association of a protein of interest with specific target loci. Alternatively, it can be combined with high-throughput sequencing technology to identify genome-wide binding sites. Here, we describe a ChIP protocol that was optimized for low-abundance transcription factors in Arabidopsis, and provide guidance on how to adapt it for other types of plants and proteins.

Keywords: Chromatin immunoprecipitation; Gene regulatory networks; Transcriptional regulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism
  • Arabidopsis* / genetics
  • Arabidopsis* / metabolism
  • Binding Sites
  • CLOCK Proteins
  • Chromatin
  • Chromatin Immunoprecipitation
  • Circadian Clocks*

Substances

  • Arabidopsis Proteins
  • Chromatin
  • CLOCK Proteins