FAM13A promotes proliferation of bovine preadipocytes by targeting Hypoxia-Inducible factor-1 signaling pathway

Adipocyte. 2021 Dec;10(1):546-557. doi: 10.1080/21623945.2021.1986327.

Abstract

The family with sequence similarity 13 member A (FAM13A) gene has been discovered in recent years and is related to metabolism. In this study, the function of FAM13A in precursor adipocyte proliferation in Qinchuan cattle was investigated using fluorescence quantitative polymerase chain reaction (PCR), western blotting, 5-ethynyl-2'-deoxyuridine staining, and other tests. FAM13A promoted precursor adipocyte proliferation. To determine the pathway FAM13A was involved in, transcriptome sequencing, fluorescence quantitative PCR, western blotting, and other tests were used, which identified the hypoxia inducible factor-1 (HIF-1) signalling pathway. Finally, cobalt chloride, a chemical mimic of hypoxia, was used to treat precursor adipocytes. mRNA and protein levels of FAM13A were significantly increased after hypoxia. Thus, FAM13A promoted bovine precursor adipocyte proliferation by inhibiting the HIF-1 signalling pathway, whereas chemically induced hypoxia negatively regulated FAM13A expression, regulating cell proliferation.

Keywords: FAM13A; hif-1 pathway; hypoxia; proliferation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipocytes
  • Animals
  • Cattle
  • Cell Proliferation
  • Hypoxia
  • Hypoxia-Inducible Factor 1*
  • Hypoxia-Inducible Factor 1, alpha Subunit
  • Signal Transduction*

Substances

  • Hypoxia-Inducible Factor 1
  • Hypoxia-Inducible Factor 1, alpha Subunit

Grants and funding

This study was supported by the National Key Research and Development Program of China (Grant No. 2018YFD0501700), China Postdoctoral Science Foundation (2020T130540), National Beef and Yak Industrial Technology System (Grant No. CARS-37), Agricultural Science and Technology Innovation and Transformation Project of Shaanxi Province (Grant No. NYKJ-2018-LY09), and the Technical Innovation Engineering Project of Shaanxi Province (Grant No. 2016KTCL02-15). We would like to thank Editage (www.editage.cn) for English language editing.