Endophytic Bacterial Communities of Ginkgo biloba Leaves During Leaf Developmental Period

Yan Deng; Haonan Huang; Fangying Lei; Shaodong Fu; Kai Zou; Shuangfei Zhang; Xueduan Liu; Luhua Jiang; Hongwei Liu; Bo Miao; Yili Liang

doi:10.3389/fmicb.2021.698703

Endophytic Bacterial Communities of Ginkgo biloba Leaves During Leaf Developmental Period

Front Microbiol. 2021 Oct 4:12:698703. doi: 10.3389/fmicb.2021.698703. eCollection 2021.

Authors

Yan Deng^{1

2}, Haonan Huang^{1

2}, Fangying Lei^{1

2}, Shaodong Fu^{1

2}, Kai Zou^{1

2}, Shuangfei Zhang^{1

2}, Xueduan Liu^{1

2}, Luhua Jiang^{1

2}, Hongwei Liu^{1

2}, Bo Miao^{1

2}, Yili Liang^{1

2}

Affiliations

¹ School of Minerals Processing and Bioengineering, Central South University, Changsha, China.
² Key Laboratory of Biometallurgy, Ministry of Education, Changsha, China.

Abstract

Plant-specialized secondary metabolites have ecological functions in mediating interactions between plants and their entophytes. In this study, high-throughput gene sequencing was used to analyze the composition and abundance of bacteria from Ginkgo leaves at five different sampling times. The results indicated that the bacterial community structure varied during leaf developmental stage. Bacterial diversity was observed to be the highest at T2 stage and the lowest at T1 stage. Proteobacteria, Firmicutes, Actinobacteria, Chloroflexi, Cyanobacteria, and Bacteroidetes were found as the dominant phyla. The major genera also showed consistency across sampling times, but there was a significant variation in their abundance, such as Bacillus, Lysinibacillus, and Staphylococcus. Significant correlations were observed between endophytic bacteria and flavonoids. Especially, Staphylococcus showed a significant positive correlation with quercetin, and changes in the abundance of Staphylococcus also showed a strong correlation with flavonoid content. In order to determine the effect of flavonoids on endophytic bacteria of Ginkgo leaves, an extracorporeal culture of related strains (a strain of Staphylococcus and a strain of Deinococcus) was performed, and it was found that the effect of flavonoids on them remained consistent. The predicted result of Tax4Fun2 revealed that flavonoids might lead to a lower abundance of endophytic microorganisms, which further proved the correlation between bacterial communities and flavonoids. This study provided the first insight into the bacterial community composition during the development of Ginkgo leaves and the correlation between the endophytic bacteria and flavonoids.

Keywords: Ginkgo biloba; bacterial endophyte communities; community assembly; flavonoids; function prediction.