Antioxidant activities of an exopolysaccharide isolated and purified from marine Pseudomonas PF-6

Shuhong Ye; Feng Liu; Jihui Wang; Han Wang; Meiping Zhang

doi:10.1016/j.carbpol.2011.08.057

Antioxidant activities of an exopolysaccharide isolated and purified from marine Pseudomonas PF-6

Carbohydr Polym. 2012 Jan 4;87(1):764-770. doi: 10.1016/j.carbpol.2011.08.057. Epub 2011 Aug 26.

Authors

Shuhong Ye¹, Feng Liu¹, Jihui Wang², Han Wang¹, Meiping Zhang¹

Affiliations

¹ School of Food Science and Technology, Dalian Polytechnic University, Liaoning Key Laboratory of Food Biotechnology, Dalian 116034, China.
² School of Food Science and Technology, Dalian Polytechnic University, Liaoning Key Laboratory of Food Biotechnology, Dalian 116034, China. Electronic address: bzixing@163.com.

PMID: 34663034
DOI: 10.1016/j.carbpol.2011.08.057

Abstract

An acidic exopolysaccharide (EPS) was isolated and purified from marine Pseudomonas PF-6. The test of physical properties indicated that the EPS was odorless, soluble in water, insoluble in organic solvents, and had a molecular weight of 8.83×10⁵Da. The test of chemical properties of the EPS showed that the EPS could be oxidized into uronic acid. Infrared spectrometry analysis exhibited absorption bands at 927.49cm^-1 and 811.76cm^-1, suggesting that the EPS belonged to a β-type heteropolysaccharide with a pyran group. Several in vitro assays were applied to evaluate the antioxidant potential of the EPS. Results showed that the acidic EPS had scavenging actions on DPPH·, ·OH and O₂^-· and the antioxidant activities increased with increases in its concentration. The scavenging action of the EPS on ·OH was stronger than that of V_c, and its activity on O₂^-· was almost the same as that of V_c.

Keywords: Antioxidant activity; Exopolysaccharides; Marine; Properties; Pseudomonas.