Structural characterization of a sulfated glucan isolated from the aqueous extract of Hedysarum polybotrys Hand.-Mazz

Yikai Shi; Lianggong Zhao; Xiaohua Liu; Fangdi Hu; Fang Cui; Yingyan Bi; Yufeng Ma; Shilan Feng

doi:10.1016/j.carbpol.2011.07.032

Structural characterization of a sulfated glucan isolated from the aqueous extract of Hedysarum polybotrys Hand.-Mazz

Carbohydr Polym. 2012 Jan 4;87(1):160-169. doi: 10.1016/j.carbpol.2011.07.032. Epub 2011 Jul 27.

Authors

Yikai Shi¹, Lianggong Zhao², Xiaohua Liu¹, Fangdi Hu¹, Fang Cui¹, Yingyan Bi¹, Yufeng Ma¹, Shilan Feng³

Affiliations

¹ School of Pharmacy, Lanzhou University, 199 Donggangxi Road, Lanzhou 730000, PR China.
² The Second Clinical Medical School of Lanzhou University, 82 Cuiying Men, Lanzhou 730030, PR China.
³ School of Pharmacy, Lanzhou University, 199 Donggangxi Road, Lanzhou 730000, PR China; Key Lab of Preclinical Study for New Drugs of Gansu Province, Lanzhou University, 199 Donggangxi Road, Lanzhou 730000, PR China. Electronic address: fengshl@lzu.edu.cn.

PMID: 34662946
DOI: 10.1016/j.carbpol.2011.07.032

Abstract

A water-soluble sulfated glucan, SHG, was isolated from Hedysarum polybotrys Hand.-Mazz using anion-exchange and gel-permeation chromatography. Elemental analysis indicated that SHG was a sulfated polysaccharide containing small amount of sulfate groups (1.47mass%). The molecular weight was estimated to be 1.72×10⁵Da by high-performance gel permeation chromatography (HPGPC) and size exclusion chromatography-multi angle laser light scattering (SEC-MALLS). SHG took random coil compact conformation in 0.1M NaNO₃. Compositional analysis revealed that SHG was composed of glucose only. On the base of partial acid hydrolysis, methylation analysis, gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared (FT-IR) spectra, nuclear magnetic resonance (NMR) spectroscopy of ¹H, ¹³C, β-correlation spectroscopy (β-COSY), total correlation spectroscopy (TOCSY), heteronuclear single quantum coherence (HSQC), and heteronuclear multiple bond correlation (HMBC), the results showed that SHG was composed of α-d-(1→4)-linked glucopyranosyl residues, with branches at C-6 consisting of non-reducing terminal approximately every eight residues. Sulfate groups may attach to the backbone at O-6, occasionally occurring per thirty-eight anhydrous glucose units.

Keywords: Hedysarum polybotrys Hand.-Mazz; NMR spectroscopy; Structure; Sulfated glucan.