Immune-mediated disease associated microbial community responded to PAH stress in phyllosphere of roadside greenspaces in Shanghai

Abstract

Microorganisms in urban greenspaces play key roles in ecosystem service provision and potentially influence human health. Increasing evidence suggests that anthropogenic disturbance poses constant stress on urban microbial communities, yet, as previous studies have focused on non-contaminated greenspaces, it has remained largely unknown how microorganisms respond to anthropogenic stress in roadside greenspaces with contamination. Our previous effort determined phyllosphere PAHs of camphor trees in 84 sites of roadside greenspaces along the urban-rural gradient in Shanghai. Here, we further investigated the phyllosphere microbial communities (PMCs) of the same sites across the same urban categories, including urban, suburban, and rural areas using high-throughput DNA sequencing. We aimed to explore how PMCs, especially those associated with immune-mediated diseases (IMDs), were affected by PAHs and the surrounding land-use types. We found that several microorganisms associated with increasing IMD risk were stimulated by PAHs. The composition of PMCs differed between the three urban categories which can be largely explained by the variation of phyllosphere PAH concentration and the surrounding land-use types. Similar to our previous study, suburban areas were linked with the most potential adverse health effects, where we observed the lowest bacterial diversity, the highest relative abundance of IMD-associated bacteria, and the highest relative abundance of Pathotroph. Urban green-blue infrastructure (GBI) was positively correlated with the diversity of PMCs, whereas urban grey infrastructure tended to homogenize PMCs. Notably, GBI also reduced the relative abundance of IMD-associated and pathogenic microbes, indicating the potential health benefits of GBI in land-use planning. Taken together, our study emphasizes the need to further investigate environmental communities in contaminated traffic environments, as human microbiomes are directly exposed to risky microorganisms.

Keywords: Green-blue infrastructure; Harmful microorganisms; Homogenization; Phyllosphere microbial community; Polycyclic aromatic hydrocarbon.