Recovery and purification of bikaverin produced by Fusarium oxysporum CCT7620

Mayra Mendonça; Marcela Colombo Dos Santos; Alana Kelyene Pereira; Taicia Pacheco Fill; Marcus Bruno Soares Forte; Juliano Lemos Bicas

doi:10.1016/j.fochx.2021.100136

Recovery and purification of bikaverin produced by Fusarium oxysporum CCT7620

Food Chem X. 2021 Oct 3:12:100136. doi: 10.1016/j.fochx.2021.100136. eCollection 2021 Dec 30.

Authors

Mayra Mendonça¹, Marcela Colombo Dos Santos¹, Alana Kelyene Pereira², Taicia Pacheco Fill², Marcus Bruno Soares Forte³, Juliano Lemos Bicas¹

Affiliations

¹ Department of Food Science, University of Campinas, Campinas, SP, Brazil.
² Department of Organic Chemistry, University of Campinas, Campinas, SP, Brazil.
³ Department of Food Engineering, University of Campinas, Campinas, SP, Brazil.

Abstract

Microbial pigments have a distinguished potential for applications in food and pharmaceutical industries, stimulating the research in this field. The present study evaluated the ideal conditions for extracting bikaverin (red pigment) from the biomass of Fusarium oxysporum CCT7620. Among the solvents tested, ethyl acetate extraction resulted in the highest bikaverin concentration and the kinetic study revealed a saturation in bikaverin concentration from 256 min on. Based on a preliminary economic study, three sequential extractions with ethyl acetate was considered the ideal protocol to recover bikaverin. After extraction, chromatographic methods were tested to purify bikaverin. The use of silica gel or Sephadex (open column) could not successfully purify bikaverin, but the semi-preparative HPLC resulted in a bikaverin-enriched fraction with a purity degree equivalent to the commercial analytical standard. This work provides relevant information regarding the extraction and purification of bikaverin, which may be useful for other downstraming processes.

Keywords: Biopigment; Chromatography; Extraction; Kinetic; Purification.