Genetic Mutations in TNFSF11 Were Associated With the Chronicity of Hepatitis C Among Chinese Han Population

Peng Huang; Yu-Qing Hou; Jing-Jing Wu; Yi-Di Wang; Xiang-Yu Ye; Feng Zang; Rong-Bin Yu; Sheng Yang

doi:10.3389/fmed.2021.743406

Genetic Mutations in TNFSF11 Were Associated With the Chronicity of Hepatitis C Among Chinese Han Population

Front Med (Lausanne). 2021 Oct 1:8:743406. doi: 10.3389/fmed.2021.743406. eCollection 2021.

Authors

Peng Huang¹, Yu-Qing Hou¹, Jing-Jing Wu², Yi-Di Wang¹, Xiang-Yu Ye¹, Feng Zang³, Rong-Bin Yu¹, Sheng Yang⁴

Affiliations

¹ Department of Epidemiology, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, China.
² Department of Environmental Health, Yangzhou Center for Disease Control and Prevention, Yangzhou, China.
³ Infection Management Office of the First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
⁴ Department of Biostatistics, School of Public Health, Nanjing Medical University, Nanjing, China.

Abstract

Background: Recently, several studies have reported that the host immune response can be related to the RANKL/RANK/OPG signaling pathway. However, the associations of TNFSF11, TNFRSF11A, and TNFRSF11B gene polymorphisms in the RANKL/RANK/OPG pathway with hepatitis C virus (HCV) infection outcomes remain unclear. Methods: In this case-control study, 768 persistent HCV infection and 503 spontaneous HCV clearance cases, and 1,259 control subjects were included. The Taman-MGB probe method was utilized to detect TNFSF11 rs9525641, TNFRSF11A rs8686340, and TNFRSF11B rs2073618 genotypes. The distribution of three single nucleotide polymorphisms (SNPs) genotypes was analyzed using stata14.0. Results: SNPs rs9525641, rs8086340, and rs2073618 genotype frequencies followed the Hardy-Weinberg natural population equilibrium (p = 0.637, 0.250, and 0.113, respectively). Also, rs9525641 was significantly associated with HCV chronicity risk in recessive (OR = 1.203, 95% CI: 1.018-1.420, p = 0.030) and additive models (OR = 1.545, 95% CI: 1.150-2.075, p = 0.004). The stratified analysis showed that rs9525641 variant genotypes were associated with HCV chronicity among people older than 50 years (OR =1.562, 95% CI: 1.079-2.262, p = 0.018), females (OR = 1.667, 95% CI: 1.145-2.429, p = 0.008), ALT <40 U/L (OR = 1.532, 95% CI: 1.074-2.286, p = 0.018), and AST < 40 U/L (OR = 1.552, 95% CI: 1.095-2.201, p = 0.014). Conclusion: TNFRSF11 rs9525641 was significantly associated with HCV chronicity in the Chinese population.

Keywords: TNFSF11; bioinformatics; chronicity; gene polymorphism; hepatitis C virus.