Preparation of ICA-loaded mPEG-ICA nanoparticles and their application in the treatment of LPS-induced H9c2 cell damage

Lin Zhou; Zhi Huang; Shanyi Yang; Jiarui Wei; Yan Xu; Lin Hu; Xinrong Guo; Limin Yuan; Zexuan Yuan; Xiaoping Yang; Xiaojun Tao; Qiufang Zhang

doi:10.1186/s11671-021-03609-9

Preparation of ICA-loaded mPEG-ICA nanoparticles and their application in the treatment of LPS-induced H9c2 cell damage

Nanoscale Res Lett. 2021 Oct 17;16(1):155. doi: 10.1186/s11671-021-03609-9.

Authors

Lin Zhou^#¹, Zhi Huang^#², Shanyi Yang², Jiarui Wei¹, Yan Xu², Lin Hu¹, Xinrong Guo^{1

3}, Limin Yuan², Zexuan Yuan², Xiaoping Yang², Xiaojun Tao⁴, Qiufang Zhang⁵

Affiliations

¹ Department of Geriatrics and General Medicine (QZ) of Affiliated Taihe Hospital, Pharmaology Department (LZ, JW, XG, QZ), School of Basic Medical Science, Hubei University of Medicine, Shiyan, 442000, Hubei, China.
² Key Laboratory of Study and Discovery of Small Targeted Molecules of Hunan Province (ZH, SY, YX, LY, ZY, XY, XT), School of Medicine, Hunan Normal University, Changsha, China.
³ Hubei Key Laboratory of Embryonic Stem Cell Research (XG), Hubei University of Medicine, Shiyan, 442000, Hubei, China.
⁴ Key Laboratory of Study and Discovery of Small Targeted Molecules of Hunan Province (ZH, SY, YX, LY, ZY, XY, XT), School of Medicine, Hunan Normal University, Changsha, China. xiaojtao@126.com.
⁵ Department of Geriatrics and General Medicine (QZ) of Affiliated Taihe Hospital, Pharmaology Department (LZ, JW, XG, QZ), School of Basic Medical Science, Hubei University of Medicine, Shiyan, 442000, Hubei, China. zqf112000@163.com.

^# Contributed equally.

Abstract

Hydrophilic polyethylene glycol monomethyl ether (mPEG) was grafted onto Icariin (ICA) by succinic anhydride to form a polyethylene glycol-Icariin (mPEG-ICA) polymer. The structure of the polymer was characterized by Fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance spectroscopy (NMR). mPEG-ICA nanoparticles loaded with ICA were prepared by physical embedding of ICA by dialysis. The particle size was determined to be (220 ± 13.7) nm, and the ζ potential was (2.30 ± 1.33) mV by dynamic light scattering (DLS). Under a transmission electron microscope (TEM), the nanoparticles were spherical, and the morphology was regular. In the medium with pH 7.4, the drug release rate of mPEG-ICA nanoparticles reached (52.80 ± 1.70)% within 72 h. At pH 6.8, the cumulative drug release of nanoparticles reached (75.66 ± 0.17)% within 48 h. Treatment of the nanoparticles with LPS-treated H9c2 cells maintained cell viability, reduced LDH release and exerted antiapoptotic effects. Moreover, ICA-loaded mPEG-ICA nanoparticles significantly decreased the mRNA expression of the myocardial inflammatory cytokines TNF-α, IL-1β and IL-6M. In conclusion, ICA-loaded mPEG-ICA nanoparticles protected against LPS-induced H9c2 cell injury.

Keywords: Dynamic light scattering (DLS); Fourier infrared spectroscopy; Icariin (ICA); Nuclear magnetic resonance spectroscopy (NMR); Polyethylene glycol monomethyl ether (mPEG).

Abstract

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