Virtual histopathology with ultraviolet scattering and photoacoustic remote sensing microscopy

Opt Lett. 2021 Oct 15;46(20):5153-5156. doi: 10.1364/OL.436136.

Abstract

Realistic label-free virtual histopathology has been a long sought-after goal not yet achieved with current methods. Here, we introduce high-resolution hematoxylin and eosin (H&E)-like virtual histology of unstained human breast lumpectomy specimen sections using ultraviolet scattering-augmented photoacoustic remote sensing microscopy. Together with a colormap-matching algorithm based on blind stain separation from a reference true H&E image, we are able to produce virtual H&E images of unstained tissues with close concordance to true H&E-stained sections, with promising diagnostic utility.

MeSH terms

  • Coloring Agents
  • Eosine Yellowish-(YS)
  • Hematoxylin
  • Humans
  • Microscopy*
  • Remote Sensing Technology*

Substances

  • Coloring Agents
  • Eosine Yellowish-(YS)
  • Hematoxylin