Sensitive surface-enhanced Raman spectroscopy (SERS) determination of nitrofurazone by β-cyclodextrin-protected AuNPs/γ-Al2O3 nanoparticles

Abstract

A novel surface-enhanced Raman spectroscopy (SERS) method for the determination of nitrofurazone was developed using AuNPs/γ-Al₂O₃ nanoparticles protected by β-cyclodextrin (β-CD) as substrate prepared in our lab. The optimum experimental conditions were obtained from single factor procedure and response surface modeling. A linear relationship (I_SERS = 508.96c + 31987.87, c: nmol L^-1, R² = 0.996) between SERS intensity and the concentration of nitrofurazone in the range of 3.3 - 667.0 nmol L^-1 was established, the limit of detection (LOD) was found at nmol L^-1 level (0.37 nmol L^-1 by 3S₀/S). The selectivity for the method was studied by the influences of foreign substances on the determination. The recoveries and RSD (n = 5) for the six meat samples were 95.1 % - 104.5% and 2.4 % - 4.8% respectively, which suggesting that the new SERS method was successfully to detecting nitrofurazone.

Keywords: Chicken; Duck; Nitrofurazone; Surface-enhanced Raman spectroscopy (SERS); β-CD/AuNPs/γ-Al(2)O(3) nanoparticles.