Detection of Mycolactone by Thin Layer Chromatography

Richard Kwamla Amewu; Thomas Spangenberg

doi:10.1007/978-1-0716-1779-3_14

Detection of Mycolactone by Thin Layer Chromatography

Methods Mol Biol. 2022:2387:131-149. doi: 10.1007/978-1-0716-1779-3_14.

Authors

Richard Kwamla Amewu¹, Thomas Spangenberg²

Affiliations

¹ Department of Chemistry, University of Ghana, Legon, Ghana. ramewu@ug.edu.gh.
² Global Health Institute of Merck, Ares Trading S.A. (a subsidiary of Merck KGaA Darmstadt Germany), Eysins, Switzerland.

PMID: 34643909
DOI: 10.1007/978-1-0716-1779-3_14

Abstract

By means of thin layer chromatography coupled to a fluorescence enhancer, a highly sensitive and operationally simple method to detect the mycolactones stemming from the human pathogen Mycobacterium ulcerans was developed and applied to various sample sources.

Keywords: Boronic acid; Fluorescence; Mycobacterium ulcerans; Mycolactone; Thin layer chromatography; UV-A.

MeSH terms

Chromatography, Thin Layer*
Humans
Macrolides
Mycobacterium ulcerans

Substances

Macrolides
mycolactone