Purification and Characterization of Extracellular Vesicles from Mycobacterium ulcerans Culture

Mélanie Foulon; Estelle Marion; Laurent Marsollier

doi:10.1007/978-1-0716-1779-3_5

Purification and Characterization of Extracellular Vesicles from Mycobacterium ulcerans Culture

Methods Mol Biol. 2022:2387:41-51. doi: 10.1007/978-1-0716-1779-3_5.

Authors

Mélanie Foulon¹, Estelle Marion², Laurent Marsollier¹

Affiliations

¹ Equipe ATOMycA, U1232 CRCINA, Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Nantes, Université d'Angers, Angers, France.
² Equipe ATOMycA, U1232 CRCINA, Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Nantes, Université d'Angers, Angers, France. Estelle.marion@inserm.fr.

PMID: 34643900
DOI: 10.1007/978-1-0716-1779-3_5

Abstract

Extracellular vesicles (EVs) from both eukaryotic and prokaryotic cells have been characterized over decades and present many biological properties. Since it has been shown that mycobacterial extracellular vesicles (MEVs) of M. ulcerans contain the macrolide toxin mycolactone, MEVs are known to be associated with the pathogenesis of mycobacteria. This chapter describes a method for purifying and characterizing vesicles from in vitro cultures of M. ulcerans. We also describe how purified vesicles can be used in cellular tests, to determine their role in the pathophysiology of M. ulcerans infection.

Keywords: Differential centrifugation; Extracellular vesicles; Mycolactone; Nano-tracking.

MeSH terms

Bacterial Toxins*
Buruli Ulcer
Extracellular Vesicles
Humans
Macrolides
Mycobacterium Infections*
Mycobacterium ulcerans*

Substances

Bacterial Toxins
Macrolides