Abstract
Skeletal muscle is a highly adaptable tissue and remodels in response to exercise training. Using short RNA sequencing, we determine the miRNA profile of skeletal muscle from healthy male volunteers before and after a 14-day aerobic exercise training regime. Among the exercise training-responsive miRNAs identified, miR-19b-3p was selected for further validation. Overexpression of miR-19b-3p in human skeletal muscle cells increases insulin signaling, glucose uptake, and maximal oxygen consumption, recapitulating the adaptive response to aerobic exercise training. Overexpression of miR-19b-3p in mouse flexor digitorum brevis muscle enhances contraction-induced glucose uptake, indicating that miR-19b-3p exerts control on exercise training-induced adaptations in skeletal muscle. Potential targets of miR-19b-3p that are reduced after aerobic exercise training include KIF13A, MAPK6, RNF11, and VPS37A. Amongst these, RNF11 silencing potentiates glucose uptake in human skeletal muscle cells. Collectively, we identify miR-19b-3p as an aerobic exercise training-induced miRNA that regulates skeletal muscle glucose metabolism.
© 2021. The Author(s).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Animals
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DNA-Binding Proteins / antagonists & inhibitors
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism
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Endosomal Sorting Complexes Required for Transport / genetics
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Endosomal Sorting Complexes Required for Transport / metabolism
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Energy Metabolism / genetics
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Exercise / physiology*
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Glucose / metabolism*
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Healthy Volunteers
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Humans
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Kinesins / genetics
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Kinesins / metabolism
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Male
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Mice
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Mice, Inbred C57BL
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MicroRNAs / genetics*
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MicroRNAs / metabolism
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Mitogen-Activated Protein Kinase 6 / genetics
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Mitogen-Activated Protein Kinase 6 / metabolism
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Muscle, Skeletal / cytology
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Muscle, Skeletal / metabolism
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Oncogene Proteins, Fusion / genetics
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Oncogene Proteins, Fusion / metabolism
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Oxygen Consumption / genetics
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Phosphorylation
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Physical Conditioning, Animal
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Protein Processing, Post-Translational*
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RNA, Small Interfering / genetics
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RNA, Small Interfering / metabolism
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Signal Transduction
Substances
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DNA-Binding Proteins
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Endosomal Sorting Complexes Required for Transport
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KIF13A protein, human
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KIF13A-RET fusion protein, human
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MIRN19 microRNA, human
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MIRN19 microRNA, mouse
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MicroRNAs
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Oncogene Proteins, Fusion
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RNA, Small Interfering
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RNF11 protein, human
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VPS37A protein, human
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Mitogen-Activated Protein Kinase 6
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Kinesins
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Glucose