Gastrointestinal biotransformation and tissue distribution of pterostilbene after long-term dietary administration in mice

Yue Sun; Qi Wang; Xian Wu; Fang Li; Mingyue Song; Minqi Wang; Xiaokun Cai; Zhengze Li; Zili Gao; Jinkai Zheng; Ce Qi; Jin Sun; Hang Xiao

doi:10.1016/j.foodchem.2021.131213

Gastrointestinal biotransformation and tissue distribution of pterostilbene after long-term dietary administration in mice

Food Chem. 2022 Mar 15:372:131213. doi: 10.1016/j.foodchem.2021.131213. Epub 2021 Sep 22.

Authors

Yue Sun¹, Qi Wang², Xian Wu³, Fang Li², Mingyue Song⁴, Minqi Wang², Xiaokun Cai², Zhengze Li², Zili Gao², Jinkai Zheng⁵, Ce Qi⁶, Jin Sun⁶, Hang Xiao⁷

Affiliations

¹ Anhui Engineering Laboratory for Agro-products Processing, School of Tea & Food Science, International Joint Laboratory on Tea Chemistry and Health Effects, Anhui Agricultural University, Hefei 230036, China; Department of Food Science, University of Massachusetts, Amherst, MA 01003, USA.
² Department of Food Science, University of Massachusetts, Amherst, MA 01003, USA.
³ Department of Food Science, University of Massachusetts, Amherst, MA 01003, USA; Department of Kinesiology, Nutrition and Health, Miami University, Oxford, OH 45056, USA.
⁴ Department of Food Science, University of Massachusetts, Amherst, MA 01003, USA; College of Food Science, South China Agricultural University, Guangzhou 510642, China.
⁵ Department of Food Science, University of Massachusetts, Amherst, MA 01003, USA; Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
⁶ Department of Food Science, University of Massachusetts, Amherst, MA 01003, USA; Institute of Nutrition and Health, Qingdao University, Qingdao 266071, China.
⁷ Department of Food Science, University of Massachusetts, Amherst, MA 01003, USA. Electronic address: hangxiao@foodsci.umass.edu.

PMID: 34638060
DOI: 10.1016/j.foodchem.2021.131213

Abstract

The metabolic fate of dietary compounds is closely related to their biological functions. Pterostilbene (PT) is a methylated stilbene found in many plant foods. Herein, we investigated gastrointestinal biotransformation and tissue distribution of PT in mice fed with 0.05% PT (w/w) for 5 weeks. PT and its major metabolites i.e. PT sulfate (PT-S), pinostilbene, pinostilbene sulfate, hydroxylated PT and hydroxylated PT sulfate were identified and quantified in the mucosa and content of the digestive tissues, blood, urine and vital organs. The results showed PT underwent demethylation, hydroxylation and conjugation in the small intestine, while the conjugated metabolites were largely deconjugated in the colon. Anaerobic fermentation with mouse cecal bacteria demonstrated the microbiota mediated deconjugation and demethylation of PT-S and PT, respectively. In conclusion, oral consumption of PT led to extensive biotransformation in mouse gastrointestinal tract and the metabolites of PT might play important roles in the bioactivity of PT.

Keywords: Biotransformation; Metabolites; Microbiota; Pinostilbene; Pterostilbene.

MeSH terms

Animals
Biotransformation
Colon / metabolism
Mice
Stilbenes* / metabolism
Tissue Distribution

Substances

Stilbenes
pterostilbene