Synergistic function of Au NPs/GeO2 nanozymes with enhanced peroxidase-like activity and SERS effect to detect choline iodide

Ruyi Tang; Xuemin Xia; Xia Zhang; Huan Jiang; Baihui Wang; Peilei Zhang; Yujin Zhang; Yanxue Tang; Yi Zhou

doi:10.1016/j.saa.2021.120467

Synergistic function of Au NPs/GeO₂ nanozymes with enhanced peroxidase-like activity and SERS effect to detect choline iodide

Spectrochim Acta A Mol Biomol Spectrosc. 2022 Feb 5:266:120467. doi: 10.1016/j.saa.2021.120467. Epub 2021 Oct 6.

Authors

Ruyi Tang¹, Xuemin Xia¹, Xia Zhang², Huan Jiang¹, Baihui Wang¹, Peilei Zhang¹, Yujin Zhang³, Yanxue Tang⁴, Yi Zhou⁵

Affiliations

¹ School of Materials Engineering, Shanghai University of Engineering Science, Shanghai 201620, China.
² School of Materials Engineering, Shanghai University of Engineering Science, Shanghai 201620, China. Electronic address: zhangxia@sues.edu.cn.
³ School of Electronic and Electrical Engineering, Shanghai University of Engineering Science, Shanghai 201620, China. Electronic address: yjzhang@sues.edu.cn.
⁴ Department of Physics, Shanghai Normal University, Shanghai 200234, China.
⁵ Key Laboratory of Infrared Imaging Materials and Detector, Shanghai Institute of Technical Physics, Chinese Academy of Science, Shanghai 200083, China.

PMID: 34637988
DOI: 10.1016/j.saa.2021.120467

Abstract

A novel Au NPs/GeO₂ nanozymes are developed as Surface-Enhanced Raman Scattering (SERS) substrates with the promising prospect for detection ChI. Herein, it is discovered that both Au NPs and GeO₂ nanozymes have peroxidase-like activity, catalyzing colorless 3,3',5,5'-tetramethylbenzidine (TMB) to produce blue TMB_ox. Interestingly, compared with single Au NPs or GeO₂ nanozymes, the Au NPs/GeO₂ nanozymes show stronger peroxidase-like activity, and significantly ameliorated SERS signal of TMB_ox. The mentioned two enhancements are ascribed to a positive synergistic function of Au NPs/GeO₂ nanozymes. Surprisingly, choline iodide (ChI) can inhibit the positive synergy in Au NPs/GeO₂ nanozymes, and slow down the reaction of TMB-H₂O₂-Au NPs/GeO₂ system. On this foundation, a new Au NPs/GeO₂ SERS technique with high sensitivity, label-free detection method of choline iodide (ChI) is established, suggesting that Au NPs/GeO₂ nanozymes have the potential application of water environment.

Keywords: Au NPs/GeO(2) nanozymes; ChI; Peroxidase-like; SERS.

MeSH terms

Choline
Gold*
Hydrogen Peroxide
Iodides
Metal Nanoparticles*
Peroxidase
Peroxidases
Spectrum Analysis, Raman

Substances

Iodides
Gold
Hydrogen Peroxide
Peroxidases
Peroxidase
Choline