Fine-tuning gene expression for improved biosynthesis of natural products: From transcriptional to post-translational regulation

Chenyi Li; Tian Jiang; Michelle Li; Yusong Zou; Yajun Yan

doi:10.1016/j.biotechadv.2021.107853

Fine-tuning gene expression for improved biosynthesis of natural products: From transcriptional to post-translational regulation

Biotechnol Adv. 2022 Jan-Feb:54:107853. doi: 10.1016/j.biotechadv.2021.107853. Epub 2021 Oct 9.

Authors

Chenyi Li¹, Tian Jiang¹, Michelle Li², Yusong Zou¹, Yajun Yan³

Affiliations

¹ School of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, GA 30602, USA.
² North Oconee High School, Bogart, GA 30622, USA.
³ School of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, GA 30602, USA. Electronic address: yajunyan@uga.edu.

Abstract

Microbial production of natural compounds has attracted extensive attention due to their high value in pharmaceutical, cosmetic, and food industries. Constructing efficient microbial cell factories for biosynthesis of natural products requires the fine-tuning of gene expressions to minimize the accumulation of toxic metabolites, reduce the competition between cell growth and product generation, as well as achieve the balance of redox or co-factors. In this review, we focus on recent advances in fine-tuning gene expression at the DNA, RNA, and protein levels to improve the microbial biosynthesis of natural products. Commonly used regulatory toolsets in each level are discussed, and perspectives for future direction in this area are provided.

Keywords: Dynamic regulation; Fine-tuning gene expression; Natural products.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Review

MeSH terms

Biological Products* / metabolism
Gene Expression
Metabolic Engineering*

Substances

Biological Products

Grants and funding

R35 GM128620/GM/NIGMS NIH HHS/United States