Evaluate the bisphenol A-induced redox state in cells, zebrafish and in vivo with a hydrogen peroxide turn-on fluorescent probe

Yan Huang; Lei Yu; Pengpeng Lu; Yinghui Wei; Lili Fu; Junjun Hou; Yunqing Wang; Xiaoyan Wang; Lingxin Chen

doi:10.1016/j.jhazmat.2021.127425

Evaluate the bisphenol A-induced redox state in cells, zebrafish and in vivo with a hydrogen peroxide turn-on fluorescent probe

J Hazard Mater. 2022 Feb 15;424(Pt B):127425. doi: 10.1016/j.jhazmat.2021.127425. Epub 2021 Oct 2.

Authors

Yan Huang¹, Lei Yu², Pengpeng Lu¹, Yinghui Wei³, Lili Fu¹, Junjun Hou³, Yunqing Wang⁴, Xiaoyan Wang⁵, Lingxin Chen⁶

Affiliations

¹ School of Pharmacy, Binzhou Medical University, Yantai 264003, China.
² Shandong Peninsula Engineering Research Center of Comprehensive Brine Utilization, Weifang University of Science and Technology, Weifang 262700, China.
³ Department of Respiratory Medicine, Binzhou Medical University Hospital, Binzhou 256603, China.
⁴ CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Shandong Key Laboratory of Coastal Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003,China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China.
⁵ School of Pharmacy, Binzhou Medical University, Yantai 264003, China; CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Shandong Key Laboratory of Coastal Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003,China. Electronic address: wangxy@yic.ac.cn.
⁶ School of Pharmacy, Binzhou Medical University, Yantai 264003, China; CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Shandong Key Laboratory of Coastal Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003,China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China. Electronic address: lxchen@yic.ac.cn.

PMID: 34634705
DOI: 10.1016/j.jhazmat.2021.127425

Abstract

Hydrogen peroxide (H₂O₂) is an important active oxygen species that plays a major role in redox balance and in physiological and pathological processes of various diseases of biological systems. As H₂O₂ is an endogenous active molecule, fluctuations in H₂O₂ content are not only affected by the state of biological system itself but also easily affected by Bisphenol A (BPA, a typical estrogenic environmental pollutant) in the external environment. Here, the near-infrared fluorescent probe Cy-NOH2 (λ_em = 750 nm) as a tool was synthesized to detect fluctuations in H₂O₂ content in cells and organisms induced by BPA. High sensitivity and excellent selectivity were found when the probe Cy-NOH2 was used to monitor endogenous H₂O₂ in vitro. In addition, the expression of H₂O₂ induced by different concentrations of BPA was able to be detected by the probe. Zebrafish and mice models were induced with different concentrations of BPA, and the H₂O₂ content showed significant increasing trends in zebrafish and livers of mice with increasing BPA concentrations. This study reveals that the probe Cy-NOH2 can be used as an effective tool to monitor the redox state in vivo under the influence of BPA, which provides a basis for clarifying the mechanisms of BPA in a variety of physiological and pathological processes.

Keywords: Bisphenol A; Environmental pollutant; Fluorescent probe; Hydrogen peroxide; Redox state.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Benzhydryl Compounds
Fluorescent Dyes*
Hydrogen Peroxide*
Mice
Oxidation-Reduction
Phenols
Zebrafish

Substances

Benzhydryl Compounds
Fluorescent Dyes
Phenols
Hydrogen Peroxide
bisphenol A