C3 plant isotopic variability in a boreal mixed woodland: implications for bison and other herbivores

Abstract

Plant isotopic baselines are critical for accurately reconstructing ancient diets and environments and for using stable isotopes to monitor ecosystem conservation. This study examines the stable carbon and nitrogen isotope compositions (δ ¹³C, δ ¹⁵N) of terrestrial C₃ plants in Elk Island National Park (EINP), Alberta, Canada, with a focus on plants consumed by grazers. EINP is located in a boreal mixed woodland ecozone close to the transition area between historic wood and plains bison habitats, and is currently home to separate herds of wood and plains bison. For this study, 165 C₃ plant samples (grasses, sedges, forbs, shrubs, and horsetail) were collected from three habitat types (open, closed, and wet) during two seasons (summer and fall). There were no statistically significant differences in the δ ¹³C or δ ¹⁵N values of grasses, sedges, shrubs and forbs. On the other hand, plant δ ¹³C and δ ¹⁵N values varied among habitats and plant parts, and the values increased from summer to fall. These results have several implications for interpreting herbivore tissue isotopic compositions: (1) consuming different proportions of grasses, sedges, shrubs, and forbs might not result in isotopic niche partitioning, (2) feeding in different microhabitats or selecting different parts of the same types of plants could result in isotopic niche partitioning, and (3) seasonal isotopic changes in herbivore tissues could reflect seasonal isotopic changes in dietary plants rather than (or in addition to) changes in animal diet or physiology. In addition, the positively skewed plant δ ¹⁵N distributions highlight the need for researchers to carefully evaluate the characteristics of their distributions prior to reporting data (e.g., means, standard deviations) or applying statistical models (e.g., parametric tests that assume normality). Overall, this study reiterates the importance of accessing ecosystem-specific isotopic baselines for addressing research questions in archaeology, paleontology, and ecology.

Keywords: Bison; Boreal; Carbon isotope; Grass; Isotopic baseline; Mammoth; Nitrogen isotope; Plant; Stable isotopes; Terrestrial.