Effects of ShenLing BaiZhu San Supplementation on Gut Microbiota and Oxidative Stress in Rats with Ulcerative Colitis

Daxing Gu; Shanshan Zhou; Lili Yao; Ying Tan; Xingzi Chi; Dayou Shi; Shining Guo; Cui Liu

doi:10.1155/2021/3960989

Effects of ShenLing BaiZhu San Supplementation on Gut Microbiota and Oxidative Stress in Rats with Ulcerative Colitis

Evid Based Complement Alternat Med. 2021 Oct 1:2021:3960989. doi: 10.1155/2021/3960989. eCollection 2021.

Authors

Daxing Gu¹, Shanshan Zhou¹, Lili Yao¹, Ying Tan¹, Xingzi Chi¹, Dayou Shi^{1

2

3}, Shining Guo^{1

2

3}, Cui Liu¹

Affiliations

¹ College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
² Guangdong Technology Research Center for Traditional Chinese Veterinary Medicine and Nature Medicine, Guangzhou 510642, China.
³ International Institute of Traditional Chinese Veterinary Medicine, Guangzhou 510642, China.

Abstract

The aim of this study was to evaluate the effect of gut microbiota and antioxidation of Shenling Baizhu San (SLBZS) as a supplement in a rat model of ulcerative colitis (UC) induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS). Acute intestinal inflammation was induced in 40 male SD rats aged 4 weeks with 100 mg/kg TNBS, and then three dosages of SLBZS (0.5 g/kg, 1 g/kg, and 1.5 g/kg) were administered for eight days, respectively. Faecal microbiome composition was assessed by 16S rRNA high-throughput sequencing. The result indicated that SLBZS could reduce the diversity of gut microbiota and increased its abundance. At the genus level, the relative abundance of SCFAs producing bacteria including Prevotella and Oscillospira increased, while the relative abundance of opportunistic pathogens including Desulfovibrio and Bilophila decreased. Meanwhile, SLBZS could improve the lesions of colon and significantly reduce the level of MPO, increase the levels of SOD and CAT in rats' serum. These findings revealed that SLBZS was effective and possessed anticolitic activities in a rat model of UC by reducing macroscopical and microscopical colon injury, enhancing antioxidant capacity, and regulating gut microbiota.