Liquid Chromatography-Mass Spectrometry for Clinical Metabolomics: An Overview

Izadora L Furlani; Estéfane da Cruz Nunes; Gisele A B Canuto; Adriana N Macedo; Regina V Oliveira

doi:10.1007/978-3-030-77252-9_10

Liquid Chromatography-Mass Spectrometry for Clinical Metabolomics: An Overview

Adv Exp Med Biol. 2021:1336:179-213. doi: 10.1007/978-3-030-77252-9_10.

Authors

Izadora L Furlani¹, Estéfane da Cruz Nunes², Gisele A B Canuto², Adriana N Macedo³, Regina V Oliveira⁴

Affiliations

¹ Núcleo de Pesquisa em Cromatografia (Separare), Department of Chemistry, Federal University of São Carlos, São Carlos, SP, Brazil.
² Department of Analytical Chemistry, Institute of Chemistry, Federal University of Bahia, Salvador, BA, Brazil.
³ Department of Chemistry, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
⁴ Núcleo de Pesquisa em Cromatografia (Separare), Department of Chemistry, Federal University of São Carlos, São Carlos, SP, Brazil. oliveirarv@ufscar.br.

PMID: 34628633
DOI: 10.1007/978-3-030-77252-9_10

Abstract

Metabolomics is a discipline that offers a comprehensive analysis of metabolites in biological samples. In the last decades, the notable evolution in liquid chromatography and mass spectrometry technologies has driven an exponential progress in LC-MS-based metabolomics. Targeted and untargeted metabolomics strategies are important tools in health and medical science, especially in the study of disease-related biomarkers, drug discovery and development, toxicology, diet, physical exercise, and precision medicine. Clinical and biological problems can now be understood in terms of metabolic phenotyping. This overview highlights the current approaches to LC-MS-based metabolomics analysis and its applications in the clinical research.

Keywords: Biomarkers; Clinical research; LC-MS; Mass analyzers; Metabolomics; Sample preparation.

MeSH terms

Chromatography, Liquid
Mass Spectrometry
Medicine*
Metabolome
Metabolomics*