Genome-wide identification and expression profiling of glutathione S-transferase family under hypoxia stress in silver sillago (Sillago sihama)

Yanyang Pan; Xinghua Lin; Fangyuan Chen; Wanida Saetan; Yang Huang; Yulei Zhang; Guangli Li; Changxu Tian

doi:10.1016/j.cbd.2021.100920

Genome-wide identification and expression profiling of glutathione S-transferase family under hypoxia stress in silver sillago (Sillago sihama)

Comp Biochem Physiol Part D Genomics Proteomics. 2021 Dec:40:100920. doi: 10.1016/j.cbd.2021.100920. Epub 2021 Sep 29.

Authors

Yanyang Pan¹, Xinghua Lin², Fangyuan Chen³, Wanida Saetan⁴, Yang Huang⁵, Yulei Zhang⁶, Guangli Li⁷, Changxu Tian⁸

Affiliations

¹ Fisheries College, Guangdong Ocean University, Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Engineering Laboratory for Mariculture Organism Breeding, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, China. Electronic address: panyanyang@stu.gdou.edu.cn.
² Fisheries College, Guangdong Ocean University, Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Engineering Laboratory for Mariculture Organism Breeding, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, China. Electronic address: linxinghua97@163.com.
³ Fisheries College, Guangdong Ocean University, Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Engineering Laboratory for Mariculture Organism Breeding, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, China. Electronic address: chenfangyuan@stu.gdou.edu.cn.
⁴ Fisheries College, Guangdong Ocean University, Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Engineering Laboratory for Mariculture Organism Breeding, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, China. Electronic address: wanidamnk62@gmail.com.
⁵ Fisheries College, Guangdong Ocean University, Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Engineering Laboratory for Mariculture Organism Breeding, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, China; Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524088, China. Electronic address: zjouhy@126.com.
⁶ Fisheries College, Guangdong Ocean University, Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Engineering Laboratory for Mariculture Organism Breeding, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, China. Electronic address: yuleizhang88@163.com.
⁷ Fisheries College, Guangdong Ocean University, Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Engineering Laboratory for Mariculture Organism Breeding, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, China; Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524088, China. Electronic address: ligl@gdou.edu.cn.
⁸ Fisheries College, Guangdong Ocean University, Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Engineering Laboratory for Mariculture Organism Breeding, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, China; Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524088, China. Electronic address: tiancx@gdou.edu.cn.

PMID: 34628157
DOI: 10.1016/j.cbd.2021.100920

Abstract

Glutathione S-transferase (GST) is an important detoxification enzyme in organisms. GSTs play an important role in responding to environmental stresses. This study aimed to identify the GST gene superfamily in silver sillago (Sillago sihama) and analyze its expression pattern under hypoxia stress. A total of 17 GST genes were identified in silver sillago. Phylogenetic analysis showed that the GST gene family contained two subgroups (cytosolic and MAPEGs), and lacked three subgroups (i.e. Pi, Kappa, and MGST2). Phylogenetic and syntenic analysis revealed that GST genes were conserved in evolution. Eight SsGSTs were significantly differentially expressed under hypoxia stress in silver sillago by RNA-seq and qRT-PCR analysis. The expression levels of SsMGST3b, SsGSTO1, SsGSTT1b and SsGSTR2 genes were significantly up-regulated after 4 h of reoxygenation in the gill tissue. In the heart tissue, the expression of SsGSTR3 was significantly up-regulated after 1 h of hypoxia while the expression levels of SsGSTT1b and SsFLAP genes were significantly down-regulated after 4 h of hypoxia. In summary, this study provides for the first time a comprehensive analysis of the GST gene superfamily of silver sillago.

Keywords: Bioinformatics; Expression profile; Glutathione S-transferase; Hypoxia stress; Sillago sihama.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Genome
Glutathione Transferase* / genetics
Hypoxia / genetics
Perciformes* / genetics
Phylogeny

Substances

Glutathione Transferase