Objective: To clarify the significance of inflammasome NLRP3 in children with immune thrombocytopenia (ITP) by detecting its changes before and after treatment.
Methods: Twenty children with ITP diagnosed and treated in Xuzhou Children's Hospital were enrolled as observation group, and 10 healthy children as control group. The mRNA levels of NLRP3, ASC, and Caspase-1 were measured by real-time quantitative PCR (RT-qPCR), the serum levels of IL-18, IL-1β, and high mobility group protein B1 (HMGB1) were detected by ELISA, and the protein level of NLRP3 was detected by Western blot.
Results: In newly diagnosed ITP children, the serum levels of IL-18, IL-1β and HMGB1 significantly decreased after treatment (P<0.05). After treatment, NLRP3, ASC, and Caspase-1 mRNA levels in peripheral blood mononuclear cells were significantly lower than those before treatment (P<0.05). NLRP3 protein expression decreased significantly after treatment.
Conclusion: Expression of NLRP3 inflammasome and downstream inflammatory factors are decrease after treatment in children with ITP, which may be used as effective prognostic markers.
题目: 炎性复合体在儿童ITP治疗前后表达的变化.
目的: 通过检测炎性复合体NLRP3在儿童免疫性血小板减少症（ITP）治疗前后的变化，明确其在ITP治疗中的意义。.
方法: 纳入20例于徐州市儿童医院确诊并治疗的ITP患儿为观察组，以10例健康体检儿童为对照组。通 过实时荧光定量PCR测定NLRP3、ASC和Caspase-1的mRNA表达量，ELISA法检测外周血血清中白细胞介素-18、白 细胞介素-1β和高迁移率蛋白B1的表达，Western blot方法检测NLRP3的表达。.
结果: 初诊ITP患儿治疗后血清白细胞介素-18、白细胞介素-1β和高迁移率蛋白B1水平明显降低（P<0.05）。ITP患儿外周血单个核细胞中NLRP3、ASC和Caspase-1治疗后mRNA水平较治疗前明显降低（P<0.05）。治疗后NLRP3蛋白表达量明显降低。.
结论: NLRP3炎性复合体及下游炎症因子在初诊ITP患儿治疗有效后明显降低，可以作为治疗有效观察指标。.