Non-targeted metabolomics characterization of Annona muricata leaf extracts with anti-angiogenic activity

Casimiro Cárdenas; José Antonio Torres-Vargas; Abel Cárdenas-Valdivia; Nuria Jurado; Ana R Quesada; Melissa García-Caballero; Beatriz Martínez-Poveda; Miguel Ángel Medina

doi:10.1016/j.biopha.2021.112263

Non-targeted metabolomics characterization of Annona muricata leaf extracts with anti-angiogenic activity

Biomed Pharmacother. 2021 Dec:144:112263. doi: 10.1016/j.biopha.2021.112263. Epub 2021 Oct 6.

Authors

Casimiro Cárdenas¹, José Antonio Torres-Vargas², Abel Cárdenas-Valdivia³, Nuria Jurado⁴, Ana R Quesada⁵, Melissa García-Caballero², Beatriz Martínez-Poveda⁶, Miguel Ángel Medina⁷

Affiliations

¹ Universidad de Málaga, Andalucía Tech, Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, E-29071 Málaga, Spain; Research Support Central Services (SCAI) of the University of Málaga, Spain.
² Universidad de Málaga, Andalucía Tech, Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, E-29071 Málaga, Spain; IBIMA (Biomedical Research Institute of Málaga), E-29071 Málaga, Spain.
³ Department of Physical Chemistry, Faculty of Sciences, University of Malaga, Malaga, Spain.
⁴ Universidad de Málaga, Andalucía Tech, Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, E-29071 Málaga, Spain.
⁵ Universidad de Málaga, Andalucía Tech, Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, E-29071 Málaga, Spain; IBIMA (Biomedical Research Institute of Málaga), E-29071 Málaga, Spain; CIBER de Enfermedades Raras (CIBERER), E-29071 Málaga, Spain.
⁶ Universidad de Málaga, Andalucía Tech, Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, E-29071 Málaga, Spain; IBIMA (Biomedical Research Institute of Málaga), E-29071 Málaga, Spain; CIBER de Enfermedades Cardiovasculares (CIBERCV), Madrid, Spain.
⁷ Universidad de Málaga, Andalucía Tech, Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, E-29071 Málaga, Spain; IBIMA (Biomedical Research Institute of Málaga), E-29071 Málaga, Spain; CIBER de Enfermedades Raras (CIBERER), E-29071 Málaga, Spain. Electronic address: medina@uma.es.

PMID: 34626933
DOI: 10.1016/j.biopha.2021.112263

Abstract

The tropical plant Annona muricata has been widely used for traditional ethnobotanic and pharmacologic applications. Extracts from different parts of this plant have been shown to have a wide range of biological activities. In the present study, we carry out a metabolomic study of both aqueous and DMSO extracts from Annona muricata leaves that has allowed us to identify 33 bioactive compounds. Furthermore, we have shown that aqueous extracts are able to inhibit endothelial cell migration and both aqueous and DMSO extracts inhibit the formation of tubule-like structures by endothelial cells cultured on Matrigel. We conclude that extracts of Annona muricata leaves have great potential as anti-angiogenic natural combinations of bioactive compounds.

Keywords: Angiogenesis; Annona muricata; Graviola; Soursop; Ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UHPLC–HRMS).

MeSH terms

Angiogenesis Inhibitors / isolation & purification
Angiogenesis Inhibitors / pharmacology*
Animals
Annona* / metabolism
Cattle
Cell Differentiation / drug effects
Cell Movement / drug effects
Cells, Cultured
Chromatography, High Pressure Liquid
Endothelial Cells / drug effects*
Metabolome
Metabolomics*
Neovascularization, Physiologic / drug effects*
Phytochemicals / isolation & purification
Phytochemicals / pharmacology*
Plant Extracts / isolation & purification
Plant Extracts / pharmacology*
Plant Leaves
Spectrometry, Mass, Electrospray Ionization

Substances

Angiogenesis Inhibitors
Phytochemicals
Plant Extracts