Antiviral iridoid glycosides from Clerodendrum myricoides

Daniel Umereweneza; Jackson T Molel; Joanna Said; Yoseph Atilaw; Théoneste Muhizi; Edward Trybala; Tomas Bergström; Adolf Gogoll; Máté Erdélyi

doi:10.1016/j.fitote.2021.105055

Antiviral iridoid glycosides from Clerodendrum myricoides

Fitoterapia. 2021 Nov:155:105055. doi: 10.1016/j.fitote.2021.105055. Epub 2021 Oct 6.

Authors

Daniel Umereweneza¹, Jackson T Molel², Joanna Said², Yoseph Atilaw³, Théoneste Muhizi⁴, Edward Trybala², Tomas Bergström², Adolf Gogoll³, Máté Erdélyi⁵

Affiliations

¹ Department of Chemistry - BMC, Uppsala University, SE-751 23 Uppsala, Sweden; Department of Chemistry, College of Science and Technology, University of Rwanda, P.O Box 3900, Kigali, Rwanda.
² Department of Infectious Diseases/Virology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, S-413 46 Gothenburg, Sweden.
³ Department of Chemistry - BMC, Uppsala University, SE-751 23 Uppsala, Sweden.
⁴ Department of Chemistry, College of Science and Technology, University of Rwanda, P.O Box 3900, Kigali, Rwanda.
⁵ Department of Chemistry - BMC, Uppsala University, SE-751 23 Uppsala, Sweden. Electronic address: mate.erdelyi@kemi.uu.se.

PMID: 34626739
DOI: 10.1016/j.fitote.2021.105055

Abstract

The methanol root extract of Clerodendrum myricoides (Hochst.) Vatke afforded two new (1, 2) and two known (3, 4) iridoid glycosides. The structures of the isolated compounds were established based on NMR, IR, UV and MS data analyses. The crude extract and the isolated constituents were assayed for antiviral activity against the human respiratory syncytial virus (RSV) in human laryngeal epidermoid carcinoma (HEp-2) cells. The crude extract inhibited RSV infectivity at EC₅₀ = 0.21 μg/ml, while it showed cytotoxicity against HEp-2 cells with CC₅₀ = 9 μg/ml. Compound 2 showed 43.2% virus inhibition at 100 μM, while compounds 1 as well as 3 and 4 had only weak antiviral and cytotoxic activities.

Keywords: Antiviral activity; Clerodendrum myricoides; Iridoid glycosides; Lamiaceae; RSV inhibitor.

MeSH terms

Antiviral Agents / isolation & purification
Antiviral Agents / pharmacology*
Cell Line, Tumor
Clerodendrum / chemistry*
Humans
Iridoid Glycosides / isolation & purification
Iridoid Glycosides / pharmacology*
Molecular Structure
Phytochemicals / isolation & purification
Phytochemicals / pharmacology
Plant Extracts
Plant Roots / chemistry
Respiratory Syncytial Virus, Human / drug effects*
Rwanda

Substances

Antiviral Agents
Iridoid Glycosides
Phytochemicals
Plant Extracts