Defining the Healthy Infant Metabolome: Liquid Chromatography Tandem-Mass Spectrometry Analysis of Dried Blood Spot Extracts from the Prospective Research on Early Determinants of Illness and Children's Health Trajectories Birth Cohort Study

William S Schleif; Robert S Harlan; Frances Hamblin; Ernest K Amankwah; Neil A Goldenberg; Raquel G Hernandez; Sara B Johnson; Shannon Reed; David R Graham

doi:10.1016/j.jpeds.2021.09.061

Defining the Healthy Infant Metabolome: Liquid Chromatography Tandem-Mass Spectrometry Analysis of Dried Blood Spot Extracts from the Prospective Research on Early Determinants of Illness and Children's Health Trajectories Birth Cohort Study

J Pediatr. 2022 Feb:241:251-256.e4. doi: 10.1016/j.jpeds.2021.09.061. Epub 2021 Oct 7.

Authors

William S Schleif¹, Robert S Harlan², Frances Hamblin³, Ernest K Amankwah⁴, Neil A Goldenberg⁵, Raquel G Hernandez⁶, Sara B Johnson⁷, Shannon Reed⁸, David R Graham⁹

Affiliations

¹ Johns Hopkins All Children's Pediatric Biorepository, Johns Hopkins All Children's Hospital, St Petersburg, FL; Pediatric Biospecimen Science Program, Johns Hopkins All Children's Institute for Clinical and Translational Research, St Petersburg, FL.
² Johns Hopkins Molecular Determinants Center and Core, Johns Hopkins All Children's Hospital, St Petersburg, FL; Johns Hopkins University School of Medicine, Baltimore, MD.
³ Clinical Coordinating Center, Johns Hopkins All Children's Institute for Clinical and Translational Research, St Petersburg, FL.
⁴ Data Coordinating Center, Johns Hopkins All Children's Institute for Clinical and Translational Research, St Petersburg, FL; Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD; Department of Oncology, Johns Hopkins University School of Medicine, St Petersburg, FL.
⁵ Pediatric Biospecimen Science Program, Johns Hopkins All Children's Institute for Clinical and Translational Research, St Petersburg, FL; Clinical Coordinating Center, Johns Hopkins All Children's Institute for Clinical and Translational Research, St Petersburg, FL; Data Coordinating Center, Johns Hopkins All Children's Institute for Clinical and Translational Research, St Petersburg, FL; Pediatric Health Equity Research Program, Johns Hopkins All Children's Institute for Clinical and Translational Research, St Petersburg, FL; Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD; Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD.
⁶ Pediatric Health Equity Research Program, Johns Hopkins All Children's Institute for Clinical and Translational Research, St Petersburg, FL; Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD.
⁷ Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD; Department of Population, Family and Reproductive Health, Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD.
⁸ Johns Hopkins Molecular Determinants Center and Core, Johns Hopkins All Children's Hospital, St Petersburg, FL.
⁹ Pediatric Biospecimen Science Program, Johns Hopkins All Children's Institute for Clinical and Translational Research, St Petersburg, FL; Johns Hopkins Molecular Determinants Center and Core, Johns Hopkins All Children's Hospital, St Petersburg, FL; Department of Anesthesia and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, MD. Electronic address: dgraham@jhmi.edu.

Abstract

Newborn screening using dried plasma spots offers preanalytical advantages over conventional cards for plasma-associated targets of interest. Herein we present dried plasma spot-based methods for measuring metabolites using a 250+ compound liquid chromatography tandem mass spectrometry library. Quality assurance reduced this library to 134, and from these, 30 compounds determined the normal newborn reference ranges.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Biomarkers / blood*
Blood Preservation / methods
Blood Preservation / standards
Chromatography, Liquid*
Dried Blood Spot Testing / methods*
Dried Blood Spot Testing / standards
Female
Humans
Infant
Infant, Newborn
Male
Metabolome*
Neonatal Screening / methods*
Neonatal Screening / standards
Prospective Studies
Reference Values
Specimen Handling / methods
Specimen Handling / standards
Tandem Mass Spectrometry*

Substances

Biomarkers

Grants and funding

R01 MD011746/MD/NIMHD NIH HHS/United States