Brucella suis Seroprevalence and Associated Risk Factors in Dogs in Eastern Australia, 2016 to 2019

Catherine C Kneipp; Kate Sawford; Kate Wingett; Richard Malik; Mark A Stevenson; Siobhan M Mor; Anke K Wiethoelter

doi:10.3389/fvets.2021.727641

Brucella suis Seroprevalence and Associated Risk Factors in Dogs in Eastern Australia, 2016 to 2019

Front Vet Sci. 2021 Sep 21:8:727641. doi: 10.3389/fvets.2021.727641. eCollection 2021.

Authors

Catherine C Kneipp¹, Kate Sawford^{2

3}, Kate Wingett⁴, Richard Malik^{5

6}, Mark A Stevenson¹, Siobhan M Mor⁷, Anke K Wiethoelter¹

Affiliations

¹ Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Melbourne, VIC, Australia.
² Kate Sawford Epidemiological Consulting Pty Ltd, Sydney, NSW, Australia.
³ Local Land Services, Braidwood, NSW, Australia.
⁴ New South Wales Department of Primary Industries, Orange, NSW, Australia.
⁵ Centre for Veterinary Education, University of Sydney, Sydney, NSW, Australia.
⁶ School of Animal and Veterinary Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia.
⁷ Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool, United Kingdom.

Abstract

Brucella suis is a zoonotic disease of feral pigs that also affects pig hunting dogs, pig hunters, veterinarians and veterinary staff. In recent years the incidence of B. suis in the eastern Australian states of New South Wales (NSW) and Queensland (QLD) has increased. A cross-sectional study was conducted to document the seroprevalence, geographical extent and risk factors for B. suis in dogs at-risk of contracting the disease. Eligible dogs were those that were known to hunt or consume feral pig meat. Dogs were enrolled through private veterinary clinics and/or directly by District Veterinarians in six regions of NSW and QLD. Blood was collected by venepuncture and tested for B. suis antibodies using the Rose Bengal Test (RBT) followed by a Complement Fixation Test (CFT) if they returned a positive RBT. Owners were invited to complete a questionnaire on the dogs' signalment, husbandry including hunting practices and locations, and any clinical signs referable to brucellosis. Of the 317 dogs included in the prevalence survey, 21 were seropositive returning a survey-adjusted true seroprevalence of 9.3 (95% CI 0.45 to 18) B. suis positive dogs per 100 dogs at-risk. True seroprevalence ranged from 0 to 24 B. suis positive dogs per 100 across eastern Australia, with the highest prevalence in central west NSW and southern QLD. Adjusted for other factors, dogs that shared a household with other seropositive dogs and those that traveled away from their home regions to hunt were more likely to be seropositive. Clinical signs at presentation were not predictive of serostatus, with seropositive and seronegative dogs equally likely to present with signs consistent with brucellosis. The results obtained from this study show that B. suis exposure is relatively common in dogs that have contact with feral pigs, with one in 10 testing seropositive. Further studies are needed to understand the progression and risk of transmission from seropositive dogs.

Keywords: Australia; Brucella suis; feral pigs; hunting dogs; zoonosis.