Sulfur dioxide promotes seed germination by modulating reactive oxygen species production in maize

Abstract

Sulfur dioxide (SO₂) is generally considered to be toxic to cells, but recent studies have shown that SO₂ has positive roles in stress defense responses in plants. However, whether SO₂ functions as a signaling molecule in the developmental process, especially in seed germination, is yet to be studied. Here, we present data supporting the role of SO₂ in seed germination and possible molecular mechanisms. SO₂ treatment significantly promoted the seed germination and seed vigor in maize. The germinating seeds treated with SO₂ treatment exhibited higher reactive oxygen species (ROS) levels and NADPH oxidase activities. Furthermore, the specific NADPH oxidase inhibitor diphenyleneiodinium (DPI) strongly inhibited ROS accumulations, and SO₂-promoted seed germination and vigor. Meanwhile, α-Amylase activity and transcripts in germinating seeds treated with SO₂ were significantly elevated. These data have demonstrated that NADPH oxidase-dependent ROS production contributes to the induction of α-Amylase activity, thereby promoting seed germination upon SO₂ exposure. SO₂ might function as a signaling molecule in plant growth and development, especially in seed germination. This study might provide a theoretical foundation for the potential exploitation of hydrated SO₂ in seed germination control in crop management.

Keywords: Maize; NADPH oxidase; Reactive oxygen species; Seed germination; Sulfur dioxide.