The molecular mechanism of the transcriptional activator SWI regulating gene ARID1B affecting swallowing dysfunction after stroke in rats

Abstract

Background: After a stroke, more than 50% of patients are suffering from dysphagia. Because the swallowing dysfunction is closely related to some neural pathways, the probing of the neuro-molecular mechanism of dysphagia is very important for future diagnosis and treatment. Methods: Our study is a typical causal study with the purpose of exploring molecular mechanisms. In this study, a rat model of dysphagia after stroke was constructed, and ARID1B overexpression plasmid was injected into the rat body through tail vein injection. The number of swallows and the swallowing response time induced by distilled water in each group of rats on the 7 th and 14 th day after modeling were detected. After 14 days of successful model establishment, the rat brain tissues were collected, part of the brainstem nucleus tractus solitarius and nucleus suspicious tissues were analyzed with a Ca²⁺ fluorescent indicator to analyze the intracellular concentration of Ca²⁺. For a part of the brainstem nucleus tractus solitarius and suspected nucleus tissues, immunohistochemistry was used to analyze the expression characteristics of genes ARID1B and TACR1 related proteins. The cerebrospinal fluid of brain tissue was collected, and the expression of gene TAC1 related protein in cerebrospinal fluid was analyzed by ELISA. For a part of the brainstem nucleus tractus solitarius and suspicious nucleus tissues, western blot was used to analyze the expression of gene SMARCA1 related protein, protein UNC80 and NALCN. Results: The detection of swallowing characteristics and the detection of intracellular Ca²⁺ concentration indicate the serious impact of stroke on swallowing function. The protein expression showed a consistent trend, which also showed that the overexpression of gene ARID1B can improve swallowing function to a certain extent. Conclusion: Due to our experiments, the molecular mechanism related to dysphagia was explored to a certain extent. At the same time, we found that the overexpression of the gene ARID1B can improve the swallowing function.