Genome-wide identification, genomic organization, and expression profiling of the CONSTANS-like (COL) gene family in petunia under multiple stresses

Khadiza Khatun; Sourav Debnath; Arif Hasan Khan Robin; Antt Htet Wai; Ujjal Kumar Nath; Do-Jin Lee; Chang-Kil Kim; Mi-Young Chung

doi:10.1186/s12864-021-08019-w

Genome-wide identification, genomic organization, and expression profiling of the CONSTANS-like (COL) gene family in petunia under multiple stresses

BMC Genomics. 2021 Oct 8;22(1):727. doi: 10.1186/s12864-021-08019-w.

Authors

Khadiza Khatun¹, Sourav Debnath², Arif Hasan Khan Robin³, Antt Htet Wai⁴, Ujjal Kumar Nath³, Do-Jin Lee⁵, Chang-Kil Kim⁶, Mi-Young Chung⁷

Affiliations

¹ Department of Biotechnology, Patuakhali Science and Technology University, Patuakhali, 8602, Bangladesh.
² Department of Biochemistry and Food Analysis, Patuakhali Science and Technology University, Patuakhali, 8602, Bangladesh.
³ Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh.
⁴ Department of Biology, Yangon University of Education, Kamayut Township, 11041, Yangon, Yangon Region, Myanmar.
⁵ Department of Agricultural Education, Sunchon National University, 255 Jungangno, Suncheon, Jeonnam, 57922, Republic of Korea.
⁶ Department of Horticulture, Kyungpook National University, Daegu, South Korea. ckkim@knu.ac.kr.
⁷ Department of Agricultural Education, Sunchon National University, 255 Jungangno, Suncheon, Jeonnam, 57922, Republic of Korea. queen@sunchon.ac.kr.

Abstract

Background: CONSTANS-like (CO-like, COL) are putative zinc-finger transcription factors known to play vital role in various plant biological processes such as control of flowering time, regulation of plant growth and development and responses to stresses. However, no systematic analysis of COL family gene regarding the plant development and stress response has been previously performed in any solanaceous crop. In the present study, a comprehensive genome-wide analysis of COL family genes in petunia has been conducted to figure out their roles in development of organs and stress response.

Results: A total of 33 COL genes, 15 PaCOL genes in P. axillaris and 18 PiCOL genes in P. inflata, were identified in petunia. Subsequently, a genome-wide systematic analysis was performed in 15 PaCOL genes. Considering the domain composition and sequence similarity the 15 PaCOL and 18 PiCOL genes were phylogenetically classified into three groups those are conserved among the flowering plants. Moreover, all of the 15 PaCOL proteins were localized in nucleus. Furthermore, differential expression patterns of PaCOL genes were observed at different developmental stages of petunia. Additionally, transcript expression of 15 PaCOL genes under various abiotic and phytohormone treatments showed their response against stresses. Moreover, several cis-elements related to stress, light-responsive, hormone signaling were also detected in different PaCOL genes.

Conclusion: The phylogenetic clustering, organ specific expression pattern and stress responsive expression profile of conserved petunia COL genes indicating their involvement in plant growth and development and stress response mechanism. This work provide a significant foundation for understanding the biological roles of petunia COL genes in plant growth, development and in stress response.

Keywords: Abiotic stresses; Constans-like gene (COL); Expression patterns; Genome-wide analysis; Petunia.

MeSH terms

Gene Expression Regulation, Plant
Genomics
Petunia* / genetics
Petunia* / metabolism
Phylogeny
Plant Proteins / genetics
Plant Proteins / metabolism
Stress, Physiological / genetics

Substances

Plant Proteins