Antiradical and Cytoprotective Properties of Allium nutans L. Honey Against CCL4-Induced Liver Damage in Rats

Shynggys Sergazy; Alexander Gulyayev; Aidana Amangeldiyeva; Ayaulym Nurgozhina; Madiyar Nurgaziyev; Zarina Shulgau; Laura Chulenbayeva; Zhanagul Khassenbekova; Almagul Kushugulova; Mohamad Aljofan

doi:10.3389/fphar.2021.687763

Antiradical and Cytoprotective Properties of Allium nutans L. Honey Against CCL4-Induced Liver Damage in Rats

Front Pharmacol. 2021 Sep 20:12:687763. doi: 10.3389/fphar.2021.687763. eCollection 2021.

Authors

Shynggys Sergazy^{1

2}, Alexander Gulyayev^{1

2}, Aidana Amangeldiyeva², Ayaulym Nurgozhina^{1

2}, Madiyar Nurgaziyev^{1

2}, Zarina Shulgau³, Laura Chulenbayeva^{1

2}, Zhanagul Khassenbekova³, Almagul Kushugulova^{1

2}, Mohamad Aljofan⁴

Affiliations

¹ National Laboratory Astana, Nazarbayev University, Nur-Sultan, Kazakhstan.
² Kazakhstan Association of Researchers of the Human Microbiome, Nur-Sultan, Kazakhstan.
³ National Center for Biotechnology, Nur-Sultan, Kazakhstan.
⁴ School of Medicine, Department of Biomedical Sciences, Nazarbayaev University, Nur-Sultan, Kazakhstan.

Abstract

The aim of this study is determine the in vitro and in vivo antiradical properties and the cytoprotective activity of Allium nutans L. honey extract. The antiradical properties of the extracts were investigated in rabbit alveolar macrophages and human foreskin fibroblast (hFFs) cells in the presence of doxorubicin, a cytotoxic substance using DPPH and ABTS assays. The cytoprotective activities were determined using 18 Wistar rats divided into three different groups, a negative control, and two other groups with experimentally induced hepatotoxicity by a single intraperitoneal injection of 50% carbon tetrachloride (CCl4) oil solution. A positive control group, received drinking water only and an experimental group that was treated with Allium nutans L. honey extracts for 7 days. In vitro treatment with Allium nutans L. honey extracts resulted in 78% reduction in radical activity in DPPH and 91.6% inhibition using the ABTS. Also, honey extracts were able to preserve 100% of cell viability in the presence of the cytotoxic, doxorubicin. Furthermore, the treatment with honey extracts resulted in a significant reduction in damage to the structure of liver tissue, as well significant reduction in the levels of ALT and AST in the experimental group compared to the control group.

Keywords: CCl4-induced liver damage; anti-radical activities; cytotoxicity; doxorubicin; flavonoid; polyphenol.