Synthetic Strategies for FRET-Enabled Carbohydrate Active Enzyme Probes

Meenakshi Singh; Michael Watkinson; Eoin M Scanlan; Gavin J Miller

doi:10.1007/978-1-0716-1685-7_12

Synthetic Strategies for FRET-Enabled Carbohydrate Active Enzyme Probes

Methods Mol Biol. 2022:2370:237-264. doi: 10.1007/978-1-0716-1685-7_12.

Authors

Meenakshi Singh¹, Michael Watkinson¹, Eoin M Scanlan², Gavin J Miller³

Affiliations

¹ Lennard-Jones Laboratories, School of Chemical and Physical Sciences, Keele University, Keele, Staffordshire, UK.
² School of Chemistry and Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, Ireland.
³ Lennard-Jones Laboratories, School of Chemical and Physical Sciences, Keele University, Keele, Staffordshire, UK. g.j.miller@keele.ac.uk.

PMID: 34611873
DOI: 10.1007/978-1-0716-1685-7_12

Abstract

Carbohydrates are an essential class of biomolecule and carbohydrate active enzymes (CAZys) catalyze their synthesis, refinement, and degradation, hence contributing an overall regulatory capacity to their underpinning physiological roles. Here we survey recent accomplishments for accessing defined carbohydrate structures, suitably equipped with FRET probe capability, followed by their utilization in studying particular classes of CAZy.

Keywords: Carbohydrate; Enzyme probe; FRET; Glycosidase.

MeSH terms

Carbohydrate Metabolism
Carbohydrates
Fluorescence Resonance Energy Transfer*

Substances

Carbohydrates