Engineering microbial metabolic energy homeostasis for improved bioproduction

Tian Tong; Xiulai Chen; Guipeng Hu; Xiao-Ling Wang; Gao-Qiang Liu; Liming Liu

doi:10.1016/j.biotechadv.2021.107841

Engineering microbial metabolic energy homeostasis for improved bioproduction

Biotechnol Adv. 2021 Dec:53:107841. doi: 10.1016/j.biotechadv.2021.107841. Epub 2021 Oct 2.

Authors

Tian Tong¹, Xiulai Chen², Guipeng Hu², Xiao-Ling Wang¹, Gao-Qiang Liu³, Liming Liu⁴

Affiliations

¹ Hunan Provincial Key Laboratory for Forestry Biotechnology, Central South University of Forestry and Technology, Changsha 410004, China; International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology of Hunan Province, Central South University of Forestry and Technology, Changsha 410004, China.
² State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China.
³ Hunan Provincial Key Laboratory for Forestry Biotechnology, Central South University of Forestry and Technology, Changsha 410004, China; International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology of Hunan Province, Central South University of Forestry and Technology, Changsha 410004, China. Electronic address: gaoliuedu@csuft.edu.cn.
⁴ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China. Electronic address: mingll@jiangnan.edu.cn.

PMID: 34610353
DOI: 10.1016/j.biotechadv.2021.107841

Abstract

Metabolic energy (ME) homeostasis is essential for the survival and proper functioning of microbial cell factories. However, it is often disrupted during bioproduction because of inefficient ME supply and excessive ME consumption. In this review, we propose strategies, including reinforcement of the capacity of ME-harvesting systems in autotrophic microorganisms; enhancement of the efficiency of ME-supplying pathways in heterotrophic microorganisms; and reduction of unessential ME consumption by microbial cells, to address these issues. This review highlights the potential of biotechnology in the engineering of microbial ME homeostasis and provides guidance for the higher efficient bioproduction of microbial cell factories.

Keywords: Energy consumption; Energy supply; Metabolic energy homeostasis; Metabolic engineering; Microbial cell factory; Synthetic biology.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Biotechnology
Homeostasis
Metabolic Engineering*
Metabolic Networks and Pathways
Synthetic Biology*