ROS/RNS and Base Dual Activatable Merocyanine-Based NIR-II Fluorescent Molecular Probe for in vivo Biosensing

Angew Chem Int Ed Engl. 2021 Dec 6;60(50):26337-26341. doi: 10.1002/anie.202109728. Epub 2021 Nov 8.

Abstract

Inflammation usually results in high-level reactive oxygen species (ROS) and reactive nitrogen species (RNS) not only in acidic tissue but also in alkaline tissue. However, noninvasively in vivo monitoring reactive species specifically within alkaline tissue remains a huge challenge. Here we introduce a dual activatable fluorescent probe PN910 located in the second near-infrared window (NIR-II, 900-1700 nm), which shows high selectivity toward H2 O2 and OONO- at pH beyond 7.4. Then we verified that PN910 could be used for the real-time, specific and accurate monitoring of cystitis and colitis for living animals. This report presents a unique approach to the development of dual activatable probe for in vivo biosensing.

Keywords: biosensing; dual activation; merocyanine; molecular probe; second near-infrared window.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Benzopyrans / chemistry*
  • Biosensing Techniques*
  • Colitis / diagnosis*
  • Colitis / metabolism
  • Cystitis / diagnosis*
  • Cystitis / metabolism
  • Fluorescent Dyes / chemistry*
  • Hydrogen Peroxide / analysis
  • Indoles / chemistry*
  • Infrared Rays
  • Mice
  • Molecular Structure
  • Nitrates / analysis
  • Reactive Nitrogen Species / metabolism
  • Reactive Oxygen Species / metabolism

Substances

  • Benzopyrans
  • Fluorescent Dyes
  • Indoles
  • Nitrates
  • Reactive Nitrogen Species
  • Reactive Oxygen Species
  • merocyanine
  • peroxynitric acid
  • Hydrogen Peroxide