Synthesis, Characterization, Immune Regulation, and Antioxidative Assessment of Yeast-Derived Selenium Nanoparticles in Cyclophosphamide-Induced Rats

Ziqian Wu; Yanli Ren; Yuejuan Liang; Liting Huang; Yuanting Yang; Ayesha Zafar; Murtaza Hasan; Fujie Yang; Xugang Shu

doi:10.1021/acsomega.1c03205

Synthesis, Characterization, Immune Regulation, and Antioxidative Assessment of Yeast-Derived Selenium Nanoparticles in Cyclophosphamide-Induced Rats

ACS Omega. 2021 Sep 14;6(38):24585-24594. doi: 10.1021/acsomega.1c03205. eCollection 2021 Sep 28.

Authors

Ziqian Wu¹, Yanli Ren¹, Yuejuan Liang¹, Liting Huang¹, Yuanting Yang¹, Ayesha Zafar², Murtaza Hasan^{1

3}, Fujie Yang¹, Xugang Shu^{1

4}

Affiliations

¹ School of Chemistry and Chemical Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, Guangdong Province, P.R. China.
² Department of Biomedical Engineering, College of Future Technology, Peking University, Beijing 100871, China.
³ Department of Biotechnology, The Institute of Biochemistry, Biotechnology and Bioinformatics, The Islamia University, Bahawalpur 63100, Pakistan.
⁴ Guangdong Province Key Laboratory of Waterfowl Healthy Breeding, Guangzhou, Guangdong Province 510225, China.

Abstract

This article introduces an environmentally friendly and more economical method for preparing red selenium nanoparticles (Se-NPs) with high stability, good biocompatibility, and narrow size using yeast as a bio-reducing agent with high antioxidant, immune regulation, and low toxicity than inorganic and organic Se. The yeast-derived Se-NPs were characterized by scanning electron microscopy, transmission electron microscopy, energy dispersive spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. The results revealed spherical-shaped particles of Se-NPs with an average diameter of 71.14 ± 18.17 nm, an amorphous structure, and surface enhancement with an organic shell layer, that provide precise geometry and stability in the formation of bio-inert gray or black Se-NPs instead of red Se-NPs. Furthermore, the addition of 0.3-0.8 mg/kg Se-NPs in the feed significantly improved the health of mice. As Se-NPs stimulated the oxidative state of mice, it significantly increased the level of GSH-Px, SOD, and AOC, and decreased the level of MDA. The yeast-derived Se-NPs alleviated the immunosuppression induced by cyclophosphamide, whereas protected the liver, spleen, and kidney of mice, stimulated the humoral immune potential of the mice, and significantly increased the levels of I g M, IgA, and I g G. These results indicated that the yeast-derived Se-NPs, as a trace element feed additive, increased the defense of the animal against oxidative stress and infectious diseases and therefore Se-NPs can be used as a potential antibiotic substitute for animal husbandry.