Osteoblastic MC3T3-E1 cells on diamond-like carbon-coated silicon plates: Field emission scanning electron microscopy data

Yoshinobu Manome; Toshiaki Tachibana; Masanori Hiratsuka; Keisuke Sato; Yasuharu Ohgoe; Kenji Hirakuri

doi:10.1016/j.dib.2021.107385

Osteoblastic MC3T3-E1 cells on diamond-like carbon-coated silicon plates: Field emission scanning electron microscopy data

Data Brief. 2021 Sep 20:38:107385. doi: 10.1016/j.dib.2021.107385. eCollection 2021 Oct.

Authors

Yoshinobu Manome¹, Toshiaki Tachibana¹, Masanori Hiratsuka², Keisuke Sato³, Yasuharu Ohgoe⁴, Kenji Hirakuri³

Affiliations

¹ Core Research Facilities, The Jikei University School of Medicine, 6-8-27 Nishi-shinbashi, Minato-ku, Tokyo, 105-8461, Japan.
² Nanotec Corporation, Nanotechno Plaza, 572-61 Toyofuta, Kashiwa City, Chiba 277-0872, Japan.
³ Department of Electrical and Electronic Engineering, Tokyo Denki University, 5 Senju Asahi-cho, Adachi-ku, Tokyo 120-8551, Japan.
⁴ Division of Electronic and Mechanical Engineering, Tokyo Denki University, Ishizaka, Hatoyama, Saitama 350-0394, Japan.

Abstract

Diamond-like carbon (DLC) is an amorphous form of carbon that contains aspects of both the diamond and graphite structures. It is composed of carbon and hydrogen, and owing to its texture, high mechanical hardness, chemical inertness, and optical transparency, DLC is widely used as a protective coating in the form of a thin film, which is applied to the surfaces of many materials. Recently, it has attracted attention as a biomedical material because of its high biocompatibility and stability [1,2]. DLC is particularly suitable to be embedded in the body owing to its low friction properties and selective cell surface attachment properties [3]. The material is currently being developed for the treatment of bone fractures [4]. However, unlike fibroblasts, the attachment of osteoblasts has not been extensively examined and no morphological data is available on how osteoblastic cells form contacts with the surface of biocompatible DLC-coated materials. Herein, such data were collected by coating DLC on the surface of silicon plates. The attachment of mouse cells to the DLC-coated plates was examined by colorimetric cell proliferation assay, and morphological observations were made using a field emission scanning electron microscope. Also, the flat cross section of the cell and plate was obtained by the ion milling method.

Keywords: Biocompatibility; Cell proliferation; Diamond like carbon; Field emission scanning electron microscopy; Osteoblastic cell.