Effects of further composting black soldier fly larvae manure on toxic metals and resistant bacteria communities by cornstalk amendment

Abstract

Rapid composting by black soldier fly larvae (BSFL) may be insufficient to maturation and humification of composting and further composting is necessary. The purpose of this study was to explore cornstalk addition on toxic metals (Cu, Zn, Pb and Cd), toxic metals resistance bacterial (TMRB) destiny and their relationship with physicochemical factors during BSFL manure composting. High-throughput sequencing was performed by six treatments, namely T1 to T6, where T1 to T3 were BSFL manures from chicken, pig and dairy manure, respectively, and T4 to T6 were same manures and utilized cornstalk to adjust C/N to 25. The results showed that cornstalk amendment could enhance the toxic metals immobilization rate compared to control treatments in the ultimate product. TMRB indicated that the major potential hosts bacteria were Firmicutes, Bacteroidota, Proteobacteria, Acidobacteriota and Actinobacteriota, and the sum relative abundance were 63.33%, 90.62%, 83.62%, 69.38%, 50.66% and 90.52% in T1 to T6 at the end of composting. Bacteria diversity and heat map revealed composting micro-ecology with additive cornstalk to remarkably effect main resistant bacterial distribution via adjusting environmental factors and potential hosts bacterial. Finally, T5 treatment was able to greatly decrease the TMRB abundance, and improve the ability of composting and ultimate product quality.

Keywords: BSFL manure; Composting; Cornstalk; Toxic metals resistance bacteria.