Utrastructure and molecular phylogeny of the myxozoan Kudoa ocellatus n. sp. (Myxozoa: Kudoidae), a parasite of the Oscar, Astronotus ocellatus (Agassiz, 1831; Teleostei: Cichlidae), a fish from northern Brazil

Abstract

Kudoa ocellatus n. sp. was found in the musculature of Astronotus ocelattus (Agassiz, 1831) from the Arari River on Marajó Island in Pará, Brazil. The new species forms pseudocysts in the epaxial and hypaxial musculature composed of various spores that are pseudoquadrate in the apical view. In the lateral view, the spores were triangular or pyramidal. In the lateral view, the spores were 46 ± 0.11 μm (4.5-4.8) in length and 6.6 ± 0.3 μm (6.2-7.2) in width, with four pyriform polar capsules of equal size that measured 2.0 ± 0.16 μm (1.8-2.2) in length and 1.5 ± 0.18 μm (1.3-1.8) in width. Based on the partial (1418 bps) sequence of the SSU rDNA gene, Kudoa ocellatus n. sp. was distinct from all the other Kudoa species deposited in GenBank. The phylogenetic Bayesian Inference and P distance placed the new species together with the other Kudoa species that parasitize freshwater Amazonian fish. The morphological evidence, together with the SSU rDNA gene sequence, supported the description of Kudoa ocellatus n. sp., a distinct new species of the genus, which parasitizes a freshwater Amazonian cichlid.

Keywords: Amazon basin; Freshwater fish; Microparasite; SSU rDNA.