Determining the Binding Kinetics of Peptide Macrocycles Using Bio-Layer Interferometry (BLI)

Katherine Rhea

doi:10.1007/978-1-0716-1689-5_19

Determining the Binding Kinetics of Peptide Macrocycles Using Bio-Layer Interferometry (BLI)

Methods Mol Biol. 2022:2371:355-372. doi: 10.1007/978-1-0716-1689-5_19.

Author

Katherine Rhea^{1

2}

Affiliations

¹ EXCET, Inc., Springfield, VA, USA. katherine.a.rhea.ctr@mail.mil.
² US Army Combat Capabilities Development Command, Chemical Biological Center (DEVCOM-CBC), Edgewood, MD, USA. katherine.a.rhea.ctr@mail.mil.

PMID: 34596858
DOI: 10.1007/978-1-0716-1689-5_19

Abstract

Bio-Layer Interferometry (BLI) enables the detection and characterization of molecular interactions in real-time without the hassle and interference of labeling. The affinity constant (K_D) obtained in the BLI analysis is an excellent indicator of quality of biomolecules such as antibodies, aptamers, peptides, etc. This method was used to analyze a peptide macrocycle against the Abrax protein, but can be used for any peptide macrocycle/analyte system.

Keywords: Affinity; Analyte; Bio-layer Interferometry; Kinetics; Label-Free Detection; Ligand; Peptides.

MeSH terms

Antibodies
Interferometry*
Kinetics
Peptides
Proteins

Substances

Antibodies
Peptides
Proteins