Discovery of Antimicrobial Peptide Macrocycles Through Bacterial Display

Justin R Randall; Cory D DuPai; Bryan W Davies

doi:10.1007/978-1-0716-1689-5_15

Discovery of Antimicrobial Peptide Macrocycles Through Bacterial Display

Methods Mol Biol. 2022:2371:287-298. doi: 10.1007/978-1-0716-1689-5_15.

Authors

Justin R Randall¹, Cory D DuPai^{1

2}, Bryan W Davies³

Affiliations

¹ Department of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA.
² Department of Integrative Biology, University of Texas at Austin, Austin, TX, USA.
³ Department of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA. bwdavies@utexas.edu.

PMID: 34596854
DOI: 10.1007/978-1-0716-1689-5_15

Abstract

Peptide macrocycles exhibit great ability to inhibit bacterial growth making them a promising new avenue for antimicrobial discovery. Surface Localized Antimicrobial Display (SLAY) is a platform allowing the high-throughput screening of large peptide libraries of diverse length, composition, or structure for their antimicrobial activity, including macrocyclic peptides cyclized through disulfide bonding. Here we describe the procedure for the design and construction of a SLAY peptide library and the process for screening that library for antimicrobial potential.

Keywords: Antimicrobial Peptides; Bacterial Pathogenesis; Macrocycles; Macrocyclic Peptides; Peptide display.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Antimicrobial Peptides / analysis*
High-Throughput Screening Assays
Peptide Library
Peptides

Substances

Antimicrobial Peptides
Peptide Library
Peptides

Grants and funding

R01 AI125337/AI/NIAID NIH HHS/United States