Determination of Mn Concentrations in Synechocystis sp. PCC6803 Using ICP-MS

Fabian Brandenburg; Hanan Schoffman; Nir Keren; Marion Eisenhut

doi:10.21769/BioProtoc.2623

Determination of Mn Concentrations in Synechocystis sp. PCC6803 Using ICP-MS

Bio Protoc. 2017 Dec 5;7(23):e2623. doi: 10.21769/BioProtoc.2623.

Authors

Fabian Brandenburg¹, Hanan Schoffman², Nir Keren², Marion Eisenhut¹

Affiliations

¹ Institute of Plant Biochemistry, Heinrich Heine University, Duesseldorf, Germany.
² Department of Plant and Environmental Sciences, Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem, Israel.

Abstract

Manganese (Mn) is an essential micronutrient for all photoautotrophic organisms. Two distinct pools of Mn have been identified in the cyanobacterium Synechocystis sp. PCC 6803 (Synechocystis), with 80% of the Mn residing in the periplasm and 20% in cytoplasm and thylakoid lumen ( Keren et al., 2002 ). In this protocol, we describe a method to quantify the periplasmic and intracellular pools of Mn in Synechocystis accurately, using inductively coupled plasma mass spectrometry (ICP-MS).

Keywords: Cyanobacteria; ICP-MS; Manganese; Periplasm; Synechocystis.