Glycosylated constituents isolated from the trunk of Abies holophylla and their anti-inflammatory and neurotrophic activity

Abstract

Eleven previously undescribed glycosylated compounds with phenolic (abeoside A-F), monoterpenyl (abeoside G and H), or 2-heptanyl (abeoside I-K) aglycone, and twenty one reported compounds were isolated from the trunk of Abies holophylla. The structures of the previously undescribed compounds were elucidated on the basis of the conventional NMR and HRMS data analysis, and the absolute configuration of sugar units were assigned by chiral derivatization and LC-MS analysis. All the isolated compounds were evaluated for their anti-neuroinflammatory and neurotrophic activities. Among the evaluated compounds, twelve compounds including abeoside A, B, E, G, H, J, and K exhibited strong anti-neuroinflammatory activities with IC₅₀ values of 4.6-18.2 μM by inhibiting production of LPS-induced NO levels, and abeoside C and 1-O-[(S)-oleuropeyl]-β-D-glucoside showed powerful effects on the stimulation of NGF secretion levels with 157.09 ± 8.53% and 154.74 ± 1.24%, respectively.

Keywords: Abies holophylla; Anti-neuroinflammation; Glycosylated natural products; Neurotrophic effect; Pinaceae.