Quantification of In Vitro Blood-Brain Barrier Permeability

Abstract

In vitro blood-brain barrier (BBB) models have been widely used to simulate in vivo models due to their low cost, feasibility, and repeatability. To serve as a valid substitute, the in vitro BBB should have the similar barrier function as that in vivo. This chapter summarizes the detailed methods for quantifying the barrier function, e.g., the permeability of the BBB to water, ions, and solutes for an in vitro BBB generated on the Transwell filter.

Keywords: Diffusive solute permeability; Hydraulic conductivity; In vitro blood-brain barrier; Trans-BBB electric resistance (TER ); Transwell filter.